Sign In | Join Free | My burrillandco.com
Home > General Assay & Diagnostic Apparatuses >

Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit

    Buy cheap Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit from wholesalers
     
    Buy cheap Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit from wholesalers
    • Buy cheap Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit from wholesalers

    Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit

    Ask Lasest Price
    Brand Name : BT Lab
    Model Number : Cat.No E0229Po
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Payment Terms : Western Union, T/T
    Supply Ability : In Stock
    Delivery Time : 1-3 business days, bulk order within one week
    • Product Details
    • Company Profile

    Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit

    Porcine Immunoglobulin M ELISA Kit with High Spesificity and Sensitivity

    Cat.No E0229Po


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine IgM antibody. IgM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine IgM Antibody is added and binds to IgM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IgM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Porcine IgM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity

    Standard Solution (24mg/ml)

    0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Porcine IgM Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Specimen Collection

    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


    Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


    Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


    Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Reagent Preparation

    All reagents should be brought to room temperature before use.

    Standard Reconstitute the 120μl of the standard (24mg/ml) with 120μl of standard diluent to generate a 12mg/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (12mg/ml) 1:2 with standard diluent to produce 6mg/ml, 3mg/ml, 1.5mg/ml and 0.75mg/ml solutions. Standard diluent serves as the zero standard(0 mg/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


    48mg/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    24mg/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    12mg/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    6mg/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    3mg/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    96mg/ml48mg/ml24mg/ml12mg/ml6mg/ml3mg/ml

    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


    Assay Procedure

    1. Prepare all reagents, standard solutions and samples as instructed. Bring all reagents to room temperature before use. The assay is performed at room temperature.

    2. Determine the number of strips required for the assay. Insert the strips in the frames for use. The unused strips should be stored at 2-8°C.

    3. Add 50μl standard to standard well. Note: Don’t add antibody to standard well because the standard solution contains biotinylated antibody.

    4. Add 40μl sample to sample wells and then add 10μl anti-MMP-3 antibody to sample wells, then add 50μl streptavidin-HRP to sample wells and standard wells (Not blank control well). Mix well. Cover the plate with a sealer. Incubate 60 minutes at 37°C.

    5. Remove the sealer and wash the plate 5 times with wash buffer. Soak wells with at least 0.35 ml wash buffer for 30 seconds to 1 minute for each wash. For automated washing, aspirate all wells and wash 5 times with wash buffer, overfilling wells with wash buffer. Blot the plate onto paper towels or other absorbent material.

    6. Add 50μl substrate solution A to each well and then add 50μl substrate solution B to each well. Incubate plate covered with a new sealer for 10 minutes at 37°C in the dark.

    7. Add 50μl Stop Solution to each well, the blue color will change into yellow immediately.

    8. Determine the optical density (OD value) of each well immediately using a microplate reader set to 450 nm within 10 minuets after adding the stop solution.


    Product Tags:

    protein elisa kit

      

    elisa test kit

      
    Quality Lab use Porcine IgM ELISA Kit , Enzyme Linked Immunosorbent Assay Kit for sale
  • Haven't found right suppliers
  • Our buyer assistants can help you find the most suitable, 100% reliable suppliers from China.
  • And this service is free of charge.
  • we have buyer assistants who speak English, French, Spanish......and we are ready to help you anytime!
  • Submit Buying Request
    Send your message to this supplier
    *From:
    Your email address is incorrect!
    *Subject:
    Your subject must be between 10-255 characters!
    *Message:
    For the best results, we recommend including the following details:
    • --Self introduction
    • --Required specifications
    • --Inquire about price/MOQ
    Your message must be between 20-3,000
    Yes! I would like your verified suppliers matching service!
    Send your message to this supplier
     
    *From:
    *To: Shanghai Korain Biotech Co., Ltd
    *Subject:
    *Message:
    Characters Remaining: (0/3000)
     
    Explore more Porcine ELISA Kit products from this supplier
    Find Similar Products By Category:
    Inquiry Cart 0

    Friendly Links: www.everychina.com

    Home| Products| Suppliers| Quality Suppliers| Site Map |About Us |Contact Us |Help |关于我们 |联系我们

    Copyright © 2009 - 2024 burrillandco.com. All rights reserved.