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Brand Name : | YaxinBio |
Model Number : | SRCPB01 |
Certification : | ISO 9001-2008 |
Payment Terms : | T/T |
Price : | 198$/100ug |
Supply Ability : | 1000g per week |
Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade
Description
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
Main Features
Purity by HPLC | ≥ 95% |
Format | Lyophilized |
Contaminant activity | No chymotrypsin, carboxypeptidase A, or other proteases contaminant. |
Application
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.
Antibody quality control.
Recommend Usage
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Storage Instructions
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.
Email:Zhaozhi@yaxinbio.com
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Company Info. |
Shanghai Yaxin Biotechnology Co.,Ltd. |
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