Lincomycin ELISA Test Kit
Product Description
REAGEN™ lincomycin ELISA Test Kit enables government agencies, food
manufacturers and quality assurance organizations to detect major
lincomycin to as low as 0.5 ppb level in various sample types and
to satisfy customer concerns about food safety. Lincomycin ELISA
Test Kit provides a unique extraction method, which is accurate,
rapid, cost effective and easy to use.
The unique features of the kit are:
1.The extraction of lincomycins from egg, honey, feed, meat, milk,
tissue, serum and urine can be finished in 10 - 30 minutes with a
high recovery rate of 75 - 105%.
2. High reproducibility.
3.A quick ELISA assay (less than 2 hours regardless of number of
samples..
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
lincomycin antibody has been coated in the plate wells. During the
analysis, sample is added along with the lincomycin-horseradish
peroxidase (Lincomycin-HRP) conjugate. If the lincomycin residue is
present in the sample, it will compete for the lincomycin antibody,
thereby preventing the Lincomycin-HRP from binding to the antibody
attached to the well. The resulting color intensity, after addition
of the HRP substrate (TMB),has an inverse relationship with the
lincomycin residue concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Egg | 1.0 |
| Honey | 4.0 |
| Feed | 1.0 |
| Meat/Liver/Kidney/Fish/Shrimp | 1.0 |
| Milk | 2.0 |
| Milk powder | 5.0 |
| Serum | 1.0 |
| Urine | 1.0 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| lincomycin | 100 |
| Clindamycin | <0.1 |
| Erythromycin | <0.1 |
| Tylosin | <0.1 |
| Apramycin | <0.1 |
| Spectinomycin | <0.1 |
| TILM | <0.1 |
