Zilpaterol ELISA Test Kit
Product Description
REAGEN™ Zilpaterol ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Zilpaterol in feed,
tissue (muscle, liver), serum and urine.
The unique features of the kit are:
1.Rapid (10 - 30 minutes), and organic reagent-free extraction
method for various samples with high recovery (80 - 95%).
2.High sensitivity (0.15 ng/g or ppb).
3.A quick ELISA assay (less than 2 hours regardless of number of
samples).
4.High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
zilpaterol Antibody has been coated in the plate wells. During the
analysis, sample and HRP-Conjugated are added to the wells. If the
target is present in the sample, it will compete for the antibody,
there by preventing the HRP-Conjugated from binding to the
zilpaterol antibody attached to the well.The resulting color
intensity, after addition of substrate, has an inverse relationship
with the target concentration in the sample
Sensitivity (Detection Limit)
| Sample Type | Detection Limit ( ppb) |
| Feed | 1.5 |
| Tissue (muscle, liver) | 0.6 |
| Serum | 0.15 |
| Urine | 0.15 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Zilpaterol | 100 |
Mabuterol | 5.8 |
| Mapenterol | 4.2 |
| Tolubuterol | 2.8 |
| Terbutaline | 2.3 |
| Cimbuterol | 1.7 |
| Salbutamol | 1.0 |
| Cimaterol | 0.6 |
| Pirbuterol | 0.4 |
| Isoprenaline | 0.2 |
1.Incubator
2.Tissue Mixer (e.g. Omni Tissue Master Homogenizer)
3.Rotary evaporator or nitrogen gas
4.Vortex mixer (e.g. Gneie Vortex mixer from VWR)
5.10, 20, 100 and 1000 mL pipettes
6Multi-channel pipette: 50-300 mL (Optional)
Warnings and Precautions
1．The standards contain Zilpaterol. Handle with particular care.
2．Do not use the kit past the expiration date.
3．Do not intermix reagents from different kits or lots except for
components with the same part No’s within their expiration dates.
ANTIBODIES AND PLATES ARE KIT-AND LOT-SPECIFIC. Make sure that the
HRP Conjugate and Diluent are mixed in correct volumes.
4．Try to maintain a laboratory temperature of 20°–25°C (68°–77°F).
Avoid running assays under or near air vents, as this may cause
excessive cooling, heating and/or evaporation. Also, do not run
assays in direct sunlight, as this may cause excessive heat and
evaporation. Cold bench tops should be avoided by placing several
layers of paper towel or some other insulate material under the
assay plates during incubation.
5．Make sure you are using only distilled or deionized water since
water quality is very important.
6．When pipetting samples or reagents into an empty microtiter
plate, place the pipette tips in the lower corner of the well,
making contact with the plastic.
7．Incubations of assay plates should be timed as precisely as
possible. Be consistent when adding standards to the assay plate.
Add your standards first and then your samples.
8．Add standards to plate only in the order from low concentration
to high concentration, as this will minimize the risk of
compromising the standard curve.
9．Always refrigerate plates in sealed bags with a desiccant to
maintain stability. Prevent condensation from forming on plates by
allowing them equilibrate to room temperature (20 – 25°C /68 –
77°F) while in the packaging.
