Pefloxacin ELISA Test Kit
Product Description
REAGEN™Pefloxacin ELISA Test Kit enables government agencies, food
manufacturers and quality assurance organizations to detect major
Pefloxacin to as low as 0.5 ppb level in various sample types and
to satisfy customer concerns about food safety. Pefloxacin ELISA
Test Kit iprovides a unique extraction method, which is accurate,
rapid, cost effective and easy to use.
The unique features of the kit are:
1.The extraction of Pefloxacin from egg, honey, feed, meat, milk,
tissue, serum and urine can be finished in 10 - 30 minutes with a
high recovery rate of 75 - 105%.
2.High reproducibility.
3.A quick ELISA assay (less than 2 hours regardless of number of
samples.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
drug of interest has been coated in the plate wells. During the
analysis, sample is added along with the primary antibody specific
for the target drug. If the target is present in the sample, it
will compete for the antibody, thereby preventing the antibody from
binding to the drug attached to the well. The secondary antibody,
tagged with a peroxidase enzyme, targets the primary antibody that
is complexed to the drug coated on the plate wells. The resulting
color intensity, after addition of substrate, has an inverse
relationship with the target concentration in the sample.
