Kanamycin ELISA Test Kit Drug Plasmid Detection With TMB Substrate

Kanamycin ELISA Test Kit- RND99033

Product Description

REAGEN^™ Kanamycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Kanamycin in meat/liver/kidney,cell lysate/culture medium/plasmid, urine, serum and milk.

The unique features of the kit are:

• High recovery (>80%), rapid (10-40 minutes), and cost-effective extraction methods.
• High sensitivity (0.2 ng/g or ppb).
• High reproducibility.
• A quick ELISA assay (less than 1 hours regardless of number of samples).

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The Kanamycin-BSA has been coated in the plate wells. During the analysis, sample and Kanamycin antibody (Antibody #1) and HRP-Conjugate (HRP-Conjugated Antibody #2) are added to the wells for incubation. If the Kanamycin residue is present in the sample, it will compete with the Kanamycin on the plate wells for the Kanamycin antibody, The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Kanamycin residue concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN^™ Kanamycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

* If you are not planning to use the kit for over 1 month, store Kanamycin Standard Stock, Kanamycin Antibody #1and HRP-Conjugated Antibody #2 at -20°C or in a freezer.