One Step H-FABP Rapid cardiac blood Tests, gold colloidal method,
diagnosis of acute myocardial infarction (AMI)
| Product | Cat No | Specimen | Sensitivity | Format | Kit Size |
| H-FABP | YC110C4 | WB / S / P | See Insert | 4mm Cassette | 25T |
Intended Use:
The H-FABP Rapid Test Cassette (Whole Blood/Serum/Plasma) is a
rapid chromatographic immunoassay for the qualitative detection of
human H-FABP in whole blood, serum or plasma as an aid in the
diagnosis of acute myocardial infarction (AMI).
Summary:
FABP is a newly introduced plasma marker of acute myocardial
infarction (AMI). The plasma kinetics of FABP (15 kD) closely
resemble those of myoglobin in that elevated plasma concentrations
are found within 2 hours after AMI and return to normal generally
within 18 to 24 hours. But the concentration of FABP in the
skeletal muscle is 20 times lower than in cardiac tissue (for
myoglobin the same content for cardiac and skeletal tissue), that
makes FABP to be more cardiac specific than myoglobin. This makes
FABP a useful biochemical marker for the early assessment or
exclusion of AMI. FABP also appears to be a useful plasma marker
for the estimation of myocardial infarct size. FABP is suitable for
use as a standard in immunoassay for early detection of acute
myocardial infarction, immunogen for antisera production, mass FABP
standard, FABP biochemical and immunochemical studies, tracer for
iodination.
Test Principle:
The H-FABP Rapid Test Cassette (Whole Blood/Serum/Plasma) is a
qualitative, membrane based immunoassay for the detection of
Heart-type Fatty Acid-Binding Protein (H-FABP) in whole blood,
serum or plasma. In this test procedure, capture reagent is
immobilized in the test line region of the test. After specimen is
added to the specimen area of the cassette, it reacts with
anti-H-FABP antibody coated colloid gold particles in the test.
This mixture migrates chromatographically along the length of the
test and interacts with the immobilized capture reagent. The test
format can detect H-FABP in specimens. If the specimen contains
Heart-type Fatty Acid-Binding Protein (H-FABP), a colored line will
appear in the test line region, indicating a positive result. If
the specimen does not contain Heart-type Fatty Acid-Binding Protein
(H-FABP), a colored line will not appear in this region, indicating
a negative result. To serve as a procedural control, a colored line
will always appear in the control line region, indicating that
proper volume of specimen has been added and membrane wicking has
occurred.
TEST PROCEDURE
1. Bring the pouched test device to room temperature(15-30℃) prior
to testing. Do not open the pouch until ready
to begin testing.
2. Remove the device from the sealed pouch and lay it on a flat and
dry surface.
3. Using the provided pipette, add one drop of fresh specimen to
the sample well.
4. Hold the buffer bottle vertically and add 1 drop to the sample
well. /If using a pipette, change a new one to avoid
cross-contamination. Draw and transfer 2-3 drops of buffer to the
sample well.
5. Read the result between 15-20minutes. Do not read results after
20 minutes.
INTERPRETATION OF RESULTS
POSITIVE:* Two lines appear. One colored line should be in the control line
region (C) and another apparent colored line should be in the test
line region (T).
*NOTE: The intensity of the color in the test line region (T) will
vary depending on the concentration of TP antibodies present in the
specimen. Therefore, any shade of color in the test line region (T)
should be considered positive.
NEGATIVE: One colored line appears in the control line region (C). No line
appears in the test line region (T).
INVALID: Control line fails to appear. Insufficient specimen volume or
incorrect procedural techniques are the most likely reasons for
control line failure. Review the procedure and repeat the test with
a new test. If the problem persists, discontinue using the test kit
immediately and contact your local distributor.
| ORIENT NEW LIFE MEDICAL CO., LTD. |
| Contact: | Jerry Meng |
| Email: | Jerry @ newlifebiotest .com |
| Tel. | +86 18657312116 |
| SKYPE | enetjerry |
