Spectinomycin rapid test strip
OVERVIEW
Brief:
The Spectinomycin rapid test strip is used for testing
Spectinomycin residue in milk qualitatively. The whole test only
need 8min, east to operate with high sensitivity.
Detection limit: 5~8ng/ml (ppb)
Principle:
The Spectinomycin rapid test strip is based on competitive
inhibition immuno-chromatographic principle. In the flow process,
Spectinomycin in the sample combined with Spectinomycin specific
colloidal gold-labeled monoclonal antibody, Inhibit the combination
between antibody and Spectinomycin-BSA conjugate on Test line of NC
membrane, lead to the color change of Test line. When the sample
has no Spectinomycin residue or concentration lower than detection
limit, T line is darker then C line; when the concentration is
equal to or higher than detection limit, T line is lighter than C
line or T line has no color or T line has same color with C line.
No matter whether there is Spectinomycin residue in sample, C line
will appear, it means the test is valid.
FEATURES & BENEFITS
Operation procedures:
1 Read the instruction carefully before use. Return test strips and
sample into room temperature.
2 Take bottles needed from the kit package, take out required
microwells, making proper marks, Please use it in 1 hour. Seal the
cap of the bottles, avoid moisture.
3 Use Micro-pipette to take 200ul of the samples into the
microwells, then repeatedly absorb for 5 times, mix the sample with
the reagent in the microwells completely until no solid judged by
eyes (this is a very important step).
4 Incubate at 40±2℃for 3 min. Then insert the strip into
micro-well, put the end with MAX down into the liquid.
5 Insert the strip into the liquid for 5min, then read the result.
Test Result Interpretation:
| Color depth comparison of T line and C line | Result judgment | Result analysis |
| T line >C line | Negative | Spectinomycinresidue is lower than detection limit in sample |
| T line =C line | Weak positive | Spectinomycin residue is equal to detection limit in sample |
| T line <C line or T line is invisible | Positive | Spectinomycin residue is higher than detection limit in sample |
| C line is invisible | Invalid | The test strip is invalid, test again |
Results picture show:
Precautions:
1) The test strip is used for once at room temperature, do not use
test strip out of date.
2) Cover the bottle immediately after taking out the reagent. If
can’t use 8 micro-wells for one time, cover the micro-wells
immediately and put back to the bottle, store in seal.
3) Do not touch the white membrane surface in the middle of test
strip, avoid sunlight and fan blowing directly.
4) Milk samples should be fresh and no polluted, for sample store
in cold and frozen should return to room temperature before
testing.
5) Use the test strips testing again for positive results.
6) Please contact the supplier for any questions.
7) The result is for reference. To confirm, please refer national
standard method.
Specificity:
This product has no cross-reaction with Quinolones, Sulfonamides
and Chloramphenicol etc.
SPECIFICATIONS
Storage and expiry date:
Storage: Store at 2-8 ℃ in sealed, dry, and dark place,no frozen.
Expiry date: 12 months; date of production is on box.
Detection limit: 5~8ng/ml (ppb)
