Tetracyclines rapid test dipsticks
OVERVIEW
Brief:
This product is used for testing Tetracyclines in milk sample (pure
milk and fresh milk) and goat’s milk sample qualitatively. The
whole test procedure only need 6min, easy to operate with high
sensitivity.
Principle:
The Tetracyclines rapid test dipstick is based on competitive
inhibition immuno-chromatographic principle. In the flow process,
Tetracyclines in the sample combined with Tetracycline specific
colloidal gold-labeled monoclonal antibody, Inhibit the combination
between antibody and Tetracyclines-BSA conjugate on Test line of NC
membrane, lead to the color change of Test line. When the sample
has no Tetracyclines residue or concentration lower than detection
limit, T line is darker than C line.When the concentration is equal
to or higher than detection limit, T line is obviously lighter than
C line or T line has no color or T line has same color with C line.
No matter whether there is Tetracyclines residue in sample, C line
will appear, it means the test is valid.
FEATURES & BENEFITS
Operation procedures:
1 Read the instruction carefully before use. Return test dipsticks
and sample into room temperature.
2 Take bottles needed from the kit package, take out required
micro-wells and dipsticks, making proper marks. Please use these
test dipsticks within 1h. Seal the cap of the bottles, avoid
moisture.
3 Take 200ul of the test samples into the micro-wells, then
repeatedly pump and suck for 5 times, mix the sample with the
reagent in the micro-wells completely until no solid judged by eyes
(this is a very important step).
4 Incubate for 3min at (40±2℃), then insert the test dipsticks into
the micro-wells with the "MAX" end fully dipped in to the mixture
solution.
5 Insert the test dipsticks into micro-wells for 3min then read the
result, it is invalid in other time.
Test Result Interpretation:
| Color depth comparison of T line and C line | Result judgment | Result analysis |
| T line >C line | Negative | Tetracyclinesresidue is lower than detection limit in sample |
| T line =C line | Weak positive | Tetracyclinesresidue is equal to detection limit in sample |
| T line <C line or T line is invisible | Positive | Tetracyclinesresidue is higher than detection limit in sample |
| C line is invisible | Invalid | The test dipstick is invalid, test again |
Results picture show:
Precautions:
1) The test dipsticks can be used only once at room temperature, do
not use test dipsticks out of expiry date.
2) Please seal the bottle after taking out required test dipsticks.
If can not use all 8 microwells at once, cup the rest and put it
back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test
card, avoid sunlight and fan blowing directly.
4) Milk samples should be fully liquid without any agglomeration
and deposition.
5) Use the test dipsticks testing again for positive results.
6) Please contact the supplier for any questions.
7) The results is for reference only, for confirmation, please
refer to the relevant national standard methods.
Specificity:
Test 500ppb Sulfonamides, quinolones, Chloramphenicol, melamine and
other drugs. All the results are negative.
SPECIFICATIONS
Storage and expiry date:
Storage: Store at 2-8 ℃ in sealed, dry, and dark place,no frozen.
Expiry date: 12 months; date of production is on box.
Detection limit in milk :
| Name | Detection limit |
| Tetracycline | 10-15 |
| Oxytetracycline | 5-10 |
| Chlortetracycline | 5-10 |
| Doxycycline | 15-20 |
