Highly sensitive Rabbit Anti Tumor Necrosis Factor Polyclonal
Antibody
Cat.No. BT-AP06654 Size: 100ul/50ul/20ul Datasheet Request
Product Information
Clonallity: Polyclonal
Applications: WB,IHC-p,IF,ELISA
Source: Rabbit
Immunogen: Synthesized peptide derived from human PDK1 around the
non-phosphorylation site of Y9
Protein Name: 3-phosphoinositide-dependent protein kinase 1
Species reactivity: human, mouse, rat
Observed band: 16 kDa
Concentration: 1 mg/ml
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium
azide
Purification:
The antibody was affinity-purified from rabbit antiserum
byaffinity-chromatography using epitope-specific immunogen.
Synonyms:
Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, TNFα, Tumor
necrosis factor
Recommended Dilution:
IF: 1: 200 - 1: 1000
IHC: 1: 100 - 1: 300
ELISA: 1: 10000
Not yet tested in other applications
Storage:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
The lyophilized antibody is stable at room temperature for at least
one month and for greater than a year when kept at -20°C.
Background
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional
proinflammatory cytokine that belongs to the tumor necrosis factor
(TNF) superfamily. It is expressed as a 26 kDa membrane bound
protein and is then cleaved by TNF-alpha converting enzyme (TACE)
to release the soluble 17 kDa monomer, which forms homotrimers in
circulation. It is produced chiefly by activated macrophages,
although it can be produced by many other cell types such as CD4+
lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and
neurons. It can bind to, and thus functions through its receptors
TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the
regulation of a wide spectrum of biological processes including
cell proliferation, differentiation, apoptosis, lipid metabolism,
and coagulation. This cytokine has been implicated in a variety of
diseases, including autoimmune diseases, insulin resistance, and
cancer.
