High Frequency Ultrasonic Liquid Processor Ultrasonic Cell Disruptor

High Frequency Ultrasonic Liquid Processor Ultrasonic Cell Disruptor

Introduction:

Ultrasonic cell disruption, also known as sonication, is a technique used in the laboratory to break down cell membranes. This is typically achieved through the use of an ultrasonic processor that generates high frequency sound waves. These sound waves create microbubbles in the liquid medium surrounding the cells, and the rapid growth and collapse of these bubbles results in shear forces that disrupt cell membranes and release intracellular contents. This technique is valuable in biotechnology and related fields for extracting cellular components, including proteins, DNA, and other biomolecules for further analysis or use.

Here's a simplified explanation of the process:

1. A sample containing cells is placed in a container (such as a test tube or beaker).

2. The container is then placed in an ultrasonic processor. The processor contains a vibrating probe, also known as a sonicator tip, which generates high-frequency sound waves.

3. These sound waves pass through the sample, creating microbubbles in the liquid medium that the cells are in.

4. The microbubbles rapidly expand and collapse, a process known as cavitation. The forces generated by this process disrupt the cell membranes.

5. The disruption of the cell membranes allows the intracellular contents to be released into the surrounding medium. These contents can then be separated, purified, and studied.

It's important to note that sonication can generate heat, which can be harmful to some cell components. Therefore, care must be taken during sonication to prevent overheating, such as using an ice bath to cool the sample or sonicating in short pulses to allow the sample to cool between rounds of sonication.

Parameter

Application:

• Cell disrupter (extraction of plant substances, disinfecting, enzyme deactivation)

• Therapeutic ultrasound, i.e. induction of thermolysis in tissues (cancer treatment)

• Decrease of reaction time and/or increase of yield

• Use of less forcing conditions e.g. lower reaction temperature

• Possible switching of reaction pathway

• Use of less or avoidance of phase transfer catalysts

• Degassing forces reactions with gaseous products

• Use of crude or technical reagents

• Activation of metals and solids

• Reduction of any induction period

• Enhancement of the reactivity of reagents or catalysts

• Generation of useful reactive species

The process of ultrasonic disruption involves several key steps:

1. Generation of Ultrasonic Waves: Ultrasonic waves are generated using a device called an ultrasonic disruptor or ultrasonic homogenizer. This device consists of a transducer that converts electrical energy into high-frequency mechanical vibrations.

2. Formation of Cavitation Bubbles: When the ultrasonic waves are transmitted to the sample, they create alternating high-pressure and low-pressure cycles. During the low-pressure cycles, small gas-filled cavities, known as cavitation bubbles, are formed within the liquid or sample. These bubbles grow rapidly during low-pressure cycles and then collapse violently during high-pressure cycles.

3. Cavitation Bubble Collapse: The collapse of cavitation bubbles generates intense localized energy in the form of shockwaves, microjets, and high temperatures. These physical forces result in the disruption or rupture of cells and tissues.

4. Mechanical and Thermal Effects: The mechanical forces generated by the collapse of cavitation bubbles cause shear stress and microstreaming within the sample. This disrupts cell membranes, breaks down cellular structures, and releases intracellular components. Additionally, the high temperatures generated during bubble collapse can also contribute to cell disruption by denaturing proteins or other heat-sensitive molecules.

5. Optimization of Parameters: The efficiency and effectiveness of ultrasonic disruption depend on various parameters, including the frequency and intensity of the ultrasonic waves, the duration of exposure, the sample volume, and the type of sample being disrupted. These parameters need to be optimized for each specific application to achieve the desired level of disruption without causing excessive damage or loss of biological activity.

R-Responsibility P-Products S-Service

sale the right products instead of the expensive one

Rps-sonic, consisted with a couple of young people who love ultrasonic very much.The founding members of RPS-SONIC have an average degree of Bachelor's degree or above. They have been in the ultrasonic industry for more than 5 years and have rich experience in ultrasound. The company's business philosophy is: Do not blindly promote any product, find the right product for the customer. So before each order, we will confirm all the details, including application details, equipment conditions, equipment specific information.

And we have the Professional import and export company， HangZhou LanBen Trade Co., Ltd, can export to any country wordwide.

Before year 2012, we only sale second branson / dukane / rinco / herrman/telsonic welding equipment , during these twenty year develop, we find, more and more people have problem with the core part of ultrasonic welding equipment -generator and transducer, so we decided to start our business of transducer and generator of our own transducer and generator. Many of the end user meet the transducer problem, they don’t know why transducer broken, and changing the expensive transducer one by one. Actually, one branson /dukane /rinco transducer can use 10~30 year, even the cheapest transducer can use about 5 years. So there must some reasons if your transducer broken in one year. That’s why we want build Rps-sonic, we need to help more end user to know more about transducer , to use ultrasonic equipment better, to save cost when meet problem.

The same to generator, Unreasonable operation may shorter the use-life of ultrasonic generator. So we have to make technical inquiry before operate ultrasonic welding machine. The key point of ultrasonic machine is resonance, only keep every part in resonance can make the system in best work environment.

Till now , we still have a lot of Branson /dukane/ rinco/ herrman telsonic welding machine, so that we can confirm every transducer/generator we made can matching to the original machine.

Of course we can make the transducer/generator replace for Branson /dukane/ rinco/ herrman telsonic welding machine, we can also make transducer/generator for any application of your ultrasonic equipment. We supply OEM service for oversea customers, we already have OEM customer in USA and German.

If you have any problem in ultrasonic area, welcome contact Rps-sonic.