High Accuracy TSH Thyrotropin Elisa Test Kit
INTENDED USE
Immunoassay for the in vitro quantitative determination of
thyrotropin in human serum.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with mouse
monoclonal Anti-TSH.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations:
0(A), 0.5(B), 2(C), 5(D), 10(E) and 25(F) μIU/mL.
• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase)
labeled mouse monoclonal Anti-TSH in Tris-NaCl buffer containing
BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine)
TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated),
PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Test principle
Sandwich principle.
Total duration of assay: 80 minutes.
• Sample, Anti-TSH coated microwells and enzyme labeled Anti-TSH
are combined. • During the incubation, TSH presents in the sample
is allowed to react simultaneously with the two antibodies,
resulting in the TSH molecules being sandwiched between the solid
phase and enzyme-linked antibodies.
• After washing, a complex is generated between the solid phase,
the TSH within the sample and enzyme-linked antibodies by
immunological reactions.
• Substrate solution is then added and catalyzed by this complex,
resulting in a chromogenic reaction. The resulting chromogenic
reaction is measured as absorbance.
• The absorbance is proportional to the amount of TSH in the
sample.
Storage
• Store at 2-8℃.
• Place unused wells in the zip-lock aluminum foiled pouch and
return to 2-8 °C, under which conditions the wells will remain
stable for 2 months, or until the labeled expiry date whichever is
earlier.
