Inhibins are polypeptides belonging to the transforming growth
factor-β (TGF-β) superfamily which also includes TGF-β, activin
glycoproteins and AMH (Anti- Müllerian hormone). All members of
this family play a role in the growth and differentiation of
tissues.
Inhibin B consists of an α-subunit and a β-subunit. Inhibin B is
produced by the sertoli cells of the testis in the male and the
granulosa
cells of the ovary in the female. Its primary role appears to be in
the regulation of gametogenesis via negative feedback on the
production
of FSH. Several published reports indicate the utility of
measurement of inhibin B as an endocrine marker for monitoring the
male1-5 and
female6-15 gonadal function.
Easy to use Ready to use reagents across whole kit, no need to
prepare reagents, only 80 minutes incubation time Wide detection
range Detection range between 1.8-1200 pg/mL and low limit of
detection up to 1.8 pg/mL Long shelf life 24 months shelf life
minimizes lot changes, which ensures long term consistency and
precision of results Automation ready Simple procedure is
automatable on most robotic platforms
Reagents Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with
25-Hydroxyvitamin D derivant
• Calibrators, 6 vials, 1 mL of each, ready to use; Concentrations:
0(A), 7.5(B),
• 15(C), 30(D), 75(E) and 150(F) ng/ml
• Incubation Buffer, 1 vial, 6.0 mL. Contains 0.2% ProClin300
preservative.
• Enzyme Conjugate, Ready to use, 1 vial, 6.0 mL of HRP
(horseradish peroxidase) labeled sheep monoclonal
Anti-25-Hydroxyvitamin D in Tris-NaCl buffer containing BSA (bovine
serum albumin). Contains 0.2% ProClin300 preservative. • Substrate,
1 vial, 11mL, ready to use, (tetramethylbenzidine) TMB
• Stop Solution, 1 vial, 6.0 mL of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 mL (40X concentrated),
PBS-Tween
• IFU, 1 copy
• Plate Lid: 1 piece.
Storage
• Store at 2-8℃
• Place unused wells in the zip-lock aluminum foiled pouch and
return to 2-8 °C, under which conditions the wells will remain
stable for 2 months, or until the labeled expiry date, whichever is
earlier.
• Seal and return unused calibrators to 2-8 °C, under which
conditions the stability will be retained for 1 month, for longer
use, store opened calibrators in aliquots and freeze at -20 °C.
Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under
which conditions the stability will be retained for 2 months, or
until the labeled
