The 25-Hydroxy Vitamin D ELISA kit is an enzymeimmunoassay intended
for the quantitative determination of total 25-hydroxy vitamin D in
human serum, plasma, tissue homogenates and other biological
fluids. Results are to be used in conjunction with other clinical
and laboratory data to assist the clinician in the assessment of
vitamin D sufficiency.
Reagents Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with
25-Hydroxyvitamin D derivant
• Calibrators, 6 vials, 1 mL of each, ready to use; Concentrations:
0(A), 7.5(B),
• 15(C), 30(D), 75(E) and 150(F) ng/ml
• Incubation Buffer, 1 vial, 6.0 mL. Contains 0.2% ProClin300
preservative.
• Enzyme Conjugate, Ready to use, 1 vial, 6.0 mL of HRP
(horseradish peroxidase) labeled sheep monoclonal
Anti-25-Hydroxyvitamin D in Tris-NaCl buffer containing BSA (bovine
serum albumin). Contains 0.2% ProClin300 preservative. • Substrate,
1 vial, 11mL, ready to use, (tetramethylbenzidine) TMB
• Stop Solution, 1 vial, 6.0 mL of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 mL (40X concentrated),
PBS-Tween
• IFU, 1 copy
• Plate Lid: 1 piece.
Storage
• Store at 2-8℃
• Place unused wells in the zip-lock aluminum foiled pouch and
return to 2-8 °C, under which conditions the wells will remain
stable for 2 months, or until the labeled expiry date, whichever is
earlier.
• Seal and return unused calibrators to 2-8 °C, under which
conditions the stability will be retained for 1 month, for longer
use, store opened calibrators in aliquots and freeze at -20 °C.
Avoid multiple freeze-thaw cycles.
• Seal and return all the other unused reagents to 2-8 °C, under
which conditions the stability will be retained for 2 months, or
until the labeled
