This H.P ELISA Test Kit is a quantitative detection of H.P antigen
in human feces, which is based on Enzyme Linked Immunosorbent
method. The kit is suitable for research of Helicobacter pylori
infection.
1. All reagents should be allowed to reach 20°C-30°C for 30 minutes
before use.
2. Dilute the wash buffer at the rate of 1:20 dilution with
distilled water before use.
3. Determine the number of holes required based on the H.P
reference material and the specimen to be tested;
4. Add 50μl H.P reference material, controls and 50μl specimen into
the corresponding wells;
5. Add 50μl conjugate into each well.
6. Shake gently to mix. Incubate in 37°C for 60 minutes with the
sealing plate membrane sealing the plate.
7. At the end of the incubation, remove and discard the plate
cover. Take out, add wash buffer to each well. Repeat wash for 5
times. After the final washing cycle, turn the plate over onto
blotting paper or clean towel, and tap it to remove any remainders.
8. Add Substrate A (50µL) and Substrate B (50µL), gently shake and
mix, and the color was developed at room temperature for 15
minutes;
9. Add 50μl Stop Solution to each well to stop the reaction.
Data processing:
1. Set the microplate reader and read the absorbance of each well
using dual wavelength
450nm/630nm.
2. Using a four-parameter fitting method, establish a standard
curve and calculate the H.P antigen content of the sample to be
teste
