Detection Kit for Estradial (E2)(ELISA).It is used in quantitative
tests for Estradial in human serum.
Product list
Product details
Description
Delivery
Within 48 hours
Packaging Specifications
8 x 12 strips, 96 wells
Country Of Origin
China
Manufacturer
18 months
Preservation method
2℃-8℃
Specimen
Whole blood
Assification
class1
Type
Elisa Test Kit
PRINCIPLE
E2 kit is based on competitive method. Coat micro well plates with E2 antibody. Add E2 calibrator (or patient sample) and HRP-E2 to form E2-antibody—HRP-E2 complex. The binding amount of HRP-E2 is reversely proportional to E2 content in serum. Remove the unbound E2-HRP. Add substrate and detect absorbent value. Calculate E2 content of patient serum through computer or plotting fitting
concentration-absorbent value curve.
MAIN COMPONENTS
Coated Microplate: 1 plate (8×12 wells), Ready to use. Coated with
anti-T4 antibody and sealed in an aluminum bag. Remove the strips
in the resealable bag with a desiccant to protect from moisture
after opened. Store at 2~8℃ until expiration date.
Biotin-T4: 1 vial of 6ml, Ready to use. Store at 2~8℃ until
expiration date.
Avidin-HRP Conjugate: 1 vial of 6ml, Ready to use. Store at 2~8℃
until expiration date.
Calibrator: 6 vials of 1ml, Ready to use. Labeled with S0 to S5 and the concentration of T4 is 0,20,40, 80,160,320 ng/ml. Store at
2~8℃ until expiration date.
Control: 2 vials of 1ml, Ready to use. Labeled with high value and
low value. Store at 2~8℃ until expiration date. The concentration
of low value is 44.3~82.3 ng/ml. The concentration of high value is
112~208 ng/ml.
Chromogen A: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
Chromogen B: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
Stop Solution: 1 vial of 7ml, Ready to use. Store at 2~8℃ until
expiration date.
Wash buffer: 1 vial of 15ml, Concentrate 20-fold, diluting with
deionized water before the assay. Store at 2~8℃ until expiration
date.
Plate sealer: 2 pieces.
Plastic resealable bag: 1 set.
Instruction manual: 1 copy.
RELATED TIPS
This kit is designated for In-Vitro Diagnostic Use Only.
Wash procedure. Incomplete washing will adversely affect the test
results. Wash each well 3 times with about 0.3ml diluted washing
solution. If no automatic washer is available, washing can be
performed manually as follows: Invert the plate vigorously to get
all water out and block the rim of well on absorbent paper for a
few seconds. Filling each well with diluted washing solution and
remain 10 seconds. Repeat these steps 3 times. Blot dry the plate
by inverting the plate onto absorbent tissue, and striking a hard
surface several times.
Drip procedure. Mix the bottle gently before use. Violent surge may
cause too much foam. Invert the bottle and squeeze one or two drop
on absorbent tissue to make sure there is no foam. Take the bottle
upright the well and make sure the drop does not touch the rim of
wells.
Storage. The whole kit should be stored at 2~8℃ for one year.
Microplate should be taken to room temperature before opened. This
is very important because absorbed atmospheric moisture by cold
plates significantly reduces their shelf life. After removing the
required number of strips, the plate should be put in the plastic
resealable bag with desiccants to minimize exposure to damp air.
Read Procedure. Determine the absorbance (OD) of each well at 450nm
with a microtiter plate reader. Using the blank well to correct the
zero point of reader if single wavelength reader is used. If double
wavelength readers with 450nm and 630nm are used, there is no need
to correct the zero point.
Control serum is prepared with human serum, which is tested
negative of HBV, HCV and HIV. But it should still be considered as
capable of transmitting viral diseases.
PREPARATIONS
1. Allow all specimens and reagents to reach room temperature
(~25°C) and mix thoroughly by gentle inversion before use. 2.Prepare Wash Buffer by diluting Wash Concentrate 20-fold with
deionized water. The diluted wash solution is stable in room
temperature for at least one week.
ATTENTIONS
1. Do not exchange reagents from different lots or use reagents
from other commercially available kits. The components of the kit
are precisely matched for optimal performance of the tests. 2. Specimens with Hyperlipidemia and icterus show no effects on the
detection results. But specimen with severe hemolysis will affect
the color of the result, other test methods are suggested to use. 3. Allow the reagents and specimens to reach room temperature
before use. Shake reagent gently before use. 4. Concentrated washing liquid will produce crystal at room
temperature, should be diluted completely before use. 5. Never reuse microplate sealing membrane. 6. Samples containing anti-sperm antibody, anti-endometrial
antibody will not affect the results. 7. Each component of the kit was inactivated, however, it should be
regarded as potentially infectious.
PRECAUTION FOR USERS
1. Handling should preclude any pipetting by mouth. Use only
pipettes with disposable tips for each specimen. 2.Do not mix materials from different master lots. Do not use kit
components beyond the expiration date. 3.All materials should be brought to room temperature before use.
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world
