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Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours

    Buy cheap Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours from wholesalers
     
    Buy cheap Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours from wholesalers
    • Buy cheap Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours from wholesalers
    • Buy cheap Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours from wholesalers

    Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours

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    Brand Name : BIOVANTION
    Model Number : TY0031
    Certification : ISO 13485
    Price : discussible
    Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram
    Supply Ability : 100000 Box/carton
    Delivery Time : 2-7 days
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    Triiodothyronine T3 ELISA Test Kit / Elisa Screening Kit Results in 2-3 hours

    Triiodothyronine(T3) ELISA Test Kit

    INTENDED USE

    Detection Kit for 3,5,3'-Triiodothyronine(T3)(Enzyme-Linked ImmunoSorbent Assay , ELISA)It is used in quantitative tests for 3,5,3’-triiodothyronine (T3) in human serum.) in human serum.

    SUMMARY AND CLINICAL SIGNIFICANCE

    The thyroid gland is essential for the development of many tissues and for the adjustement of their metabolism. These processes are under the control of thyroid hormones, T4 and T3.

    Under stimulation by pituitary thyrotropin(TSH). the thyroid gland essentially secretes T4 with minute amounts of T3.

    Tissue recptors to thyroid hormones are T3 receptors, T3 represents thus the acive entity among thyroid secretions.

    The bulk of circulating T3 is produced by peripheral deiodination of T4 alternatively, T4 yields reverse T3(or RT3), a poorly active thyronine.

    In the blood,T3 circulates free and bound to the same carrier protein transporting T4, thyroxine binding globulin or TBG. Nevertheless, T3 binding affinity to TBG is significantly less than that of T4; The free T3 serum concentration represents about 0.3% of the total circulating T3 concentration.

    Under normal conditions, total T3 level is negatively. Correlated to serum TSH level due to the negative feed -back mechanism regulating thyroid funcion.

    Product list

    Product detailsDescription
    DeliveryWithin 48 hours
    Packaging Specifications8 x 12 strips, 96 wells
    Country Of OriginChina
    Manufacturer18 months
    Preservation method2℃-8℃
    SpecimenWhole blood
    Assificationclass1
    TypeElisa Test Kit

    PRINCIPLE

    T3 kit is based on competitive method. Coat micro well plates with anti-T3 to form solid-phase antibody. The T3 of sample and biotin-T3 compet with anti-T3 on solid-phase. Vidin-HRP added, solid phase antibody-biotinylated T3-avidin-HRP complex was formed. Add substrate and detect absorbent value. Calculate T3 content of patient serum through computer or plotting fitting concentration-absorbent value curve.

    MAIN COMPONENTS

    • Coated Microplate: 1 plate (8×12 wells), Ready to use. Coated with anti-T3 antibody and sealed in an aluminum bag. Remove the strips in the resealable bag with a desiccant to protect from moisture after opened. Store at 2~8℃ until expiration date.
    • Biotin-T3: 1 vial of 6ml, Ready to use. Store at 2~8℃ until expiration date.
    • Avidin-HRP Conjugate: 1 vial of 6ml, Ready to use. Store at 2~8℃ until expiration date.
    • Calibrator: 6 vials of 1ml, Ready to use. Labeled with S0 to S5 and the concentration of T3 is 0,0.5,1.0,2.0,4.0,8.0 ng/ml. Store at 2~8℃ until expiration date.
    • Control: 2 vials of 1ml, Ready to use. Store at 2~8℃ until expiration date. The concentration of low value is 0.63~1.17ng/ml. The concentration of high value is 2.8~5.2 ng/ml.
    • Chromogen A: 1 vial of 7ml, Ready to use. Store at 2~8℃ until expiration date.
    • Chromogen B: 1 vial of 7ml, Ready to use. Store at 2~8℃ until expiration date.
    • Stop Solution: 1 vial of 7ml, Ready to use. Store at 2~8℃ until expiration date.
    • Wash buffer: 1 vial of 15ml, Concentrate 20-fold, diluting with deionized water before the assay. Store at 2~8℃ until expiration date.
    • Plate sealer: 2 pieces.
    • Plastic resealable bag: 1 set.
    • Instruction manual: 1 copy.

    RELATED TIPS

    • This kit is designated for In-Vitro Diagnostic Use Only.
    • Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with water and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
    • Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
    • Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.

    Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be


    PREPARATIONS

    1. Allow all specimens and reagents to reach room temperature (~25°C) and mix thoroughly by gentle inversion before use.

    2.Prepare Wash Buffer by diluting Wash Concentrate 20-fold with deionized water. The diluted wash solution is stable in room temperature for at least one week.

    ATTENTIONS

    • This kit is designated for In-Vitro Diagnostic Use Only.
    • Wash procedure. Incomplete washing will adversely affect the test results. Wash each well 3 times with about 0.3ml wash buffer. If no automatic washer is available, washing can be performed manually as follows: Invert the plate vigorously to get all wash buffer out and block the rim of well on absorbent paper for a few seconds. Filling each well with water and remain 10 seconds. Repeat these steps 3 times. Blot dry the plate by inverting the plate onto absorbent tissue, and striking a hard surface several times.
    • Drip procedure. Mix the bottle gently before use. Violent surge may cause too much foam. Invert the bottle and squeeze one or two drop on absorbent tissue to make sure there is no foam. Take the bottle upright the well and make sure the drop does not touch the rim of wells.
    • Read procedure. Using the blank well to correct the zero point of reader if single wavelength reader is used. If double wavelength readers with 450nm and 630nm are used, there is no need to correct the zero point.
    • Storage. The whole kit should be stored at 2~8℃ for one year. Microplate should be taken to room temperature before opened. This is very important because absorbed atmospheric moisture by cold plates significantly reduces their shelf life. After removing the required number of strips, the plate should be put in the plastic resealable bag with

    desiccants to minimize exposure to damp air.

    • Control serum is prepared with human serum, which is tested negative of HBV, HCV and HIV. But it should still be considered as capable of transmitting viral diseases.

    PRECAUTION FOR USERS

    1. Handling should preclude any pipetting by mouth. Use only pipettes with disposable tips for each specimen.

    2.Do not mix materials from different master lots. Do not use kit components beyond the expiration date.

    3.All materials should be brought to room temperature before use.


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