Features of TSH Elisa Kit:
1. Sensitive
2. Accurate
3. High quality
4. Economic price
5. Reliable and Easy to Use
Intended use:
• Immunoassay for the in vitro quantitative determination of
thyrotropin in human serum.
Reagents Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with mouse
monoclonal Anti-TSH.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations:
0(A), 0.5(B), 2(C), 5(D), 10(E) and 25(F) μIU/mL.
• Enzyme Conjugate, 1 vial, 6.0 ml of HRP (horseradish peroxidase)
labeled mouse monoclonal Anti-TSH in Tris-NaCl buffer containing
BSA (bovine serum albumin). Contains 0.1% ProClin300 preservative.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine)
TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated),
PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent
capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with
a precision of better than 1.5%.
• Absorbent paper.
• Distilled water
Precision
Precision was determined using reagents, pooled human sera, and
controls in a modifified protocol (EP5-A) of the CLSI (Clinical and
Laboratory Standards Institute): 2 times daily for 20 days (n =
40). The following results were obtained
