Human Vascular Cell Adhesion Molecule 1;VCAM1;CD106;ELISA Kit
For the quantitative in vitro determination of Vascular Cell
Adhesion Molecule 1 (VCAM1/CD106)concentrations in Human serum,
plasma, culture media or any biological fluid.
For research use only.
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa
stripplate provided in this kit has been pre-coated with an
antibody specific to VCAM1/CD106 . Standards or samples are added
to the appropriate Microelisa stripplate wells and combined to the
specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated
antibody specific for VCAM1/CD106 is added to each Microelisa
stripplate well and incubated. Free components are washed away. The
TMB substrate solution is added to each well. Only those wells that
contain VCAM1/CD106 and HRP conjugated VCAM1/CD106 antibody will
appear blue in color and then turn yellow after the addition of the
stop solution. The optical density (OD) is measured
spectrophotometrically at a wavelength of 450 nm. The OD value is
proportional to the concentration of VCAM1/CD106 . You can
calculate the concentration of VCAM1/CD106 in the samples by
comparing the OD of the samples to the standard curve.
Materials provided with the kit
Materials provided with the kit
48 determinations
96 determinations
Storage
1
User manual
1
1
R.T.
2
Closure plate membrane
2
2
R.T.
3
Sealed bags
1
1
R.T.
4
Microelisa stripplate
1
1
2-8℃
5
Standard:13.5ng/ml
0.5ml×1 bottle
0.5ml×1 bottle
2-8℃
6
Standard diluent
1.5ml×1 bottle
1.5ml×1 bottle
2-8℃
7
HRP-Conjugate reagent
3ml×1 bottle
6ml×1 bottle
2-8℃
8
Sample diluent
3ml×1 bottle
6ml×1 bottle
2-8℃
9
Chromogen Solution A
3ml×1 bottle
6ml×1 bottle
2-8℃
10
Chromogen Solution B
3ml×1 bottle
6ml×1 bottle
2-8℃
11
Stop Solution
3ml×1 bottle
6ml×1 bottle
2-8℃
12
Wash solution
20ml (20X)×1bottle
20ml (30X)×1bottle
2-8℃
Notes:
Store the kit at 4°C upon receipt.The kit should be equilibrated to
room temperature before the assay. Remove any unneeded strips from
Human VCAM1/CD106Antibody-Coated plate, reseal them in zip-lock
foil and keep at 4°C.
Calculation of Results
Known concentrations of Human VCAM1/CD106 Standard and its
corresponding reading OD is plotted on the log scale (x-axis) and
the log scale (y-axis) respectively. The concentration of Human
VCAM1/CD106 in sample is determined by plotting the sample’s O.D.
on the Y-axis. The original concentration is calculated by
multiplying the dilution factor.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with
low, middle and high level Human VCAM1/CD106 were tested 20 times
on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with
low, middle and high level Human VCAM1/CD106 were tested on 3
different plates, 8 replicates in each plate.
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world
