25-OH Vitamin D Total Elisa Kit
25OH Vit.D Total
25-Hydroxyvitamin D Total
REF: DS167701 96 tests
Intended use
The 25-Hydroxy Vitamin D ELISA kit is an enzymeimmunoassay intended
for the quantitative determination of total 25-hydroxy vitamin D in
human serum, plasma, tissue homogenates and other biological
fluids. Results are to be used in conjunction with other clinical
and laboratory data to assist the clinician in the
assessment of vitamin D sufficiency.
Summary
Vitamin D is a fat-soluble steroid hormone precursor that is mainly
produced in the skin by exposure to sunlight. Vitamin D is
biologically inert and must undergo
hydroxylation steps to become active.(1)
Our body can only synthesize vitamin D3. Vitamin D2 is taken up
with fortified food or given by supplements. Physiologically,
vitamin D3 and D2 are bound to the vitamin D-binding protein (VDBP)
in plasma and transported to the liver to become 25-hydroxyvitamin
D (vitamin D (25-OH)).
As vitamin D (25-OH) represents the major storage form, its blood
concentration is used to assess the overall vitamin D status. More
than 95 % of vitamin D (25-OH), measurable in serum, is vitamin D3
(25-OH) whereas vitamin D2 (25-OH) reaches measurable levels only
in patients taking vitamin D2 supplements.(1,2,3)
Vitamin D is essential for bone health. In children, severe
deficiency leads to rickets. In elderly, the risk of falling has
been attributed to vitamin D deficiency due
to muscle weakness. Moreover, low vitamin D (25-OH) concentrations
are ssociated with lower bone mineral density. Insufficiency has
also been linked to
diabetes, cancer, cardiovascular disease, and autoimmune
diseases.(1) The 25-OH Vitamin D is intended for the quantitative
determination of vitamin D (25-OH) in human serum, plasma, tissue
homogenates and other biological fluids, as an aid in the
assessment of vitamin D sufficiency.
Reagents
Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with
25-Hydroxyvitamin D derivant.
• Calibrators, 6 vials, 1 mL of each, ready to use; Concentrations:
0(A), 7.5(B),
• 15(C), 30(D), 75(E) and 150(F) ng/ml.
• Incubation Buffer, 1 vial, 6.0 mL. Contains 0.2% ProClin300
preservative.
• Enzyme Conjugate, Ready to use, 1 vial, 6.0 mL of HRP
(horseradish peroxidase) labeled sheep monoclonal
Anti-25-Hydroxyvitamin D in Tris-NaCl buffer containing BSA (bovine
serum albumin). Contains 0.2% ProClin300 preservative.
• Substrate, 1 vial, 11mL, ready to use, (tetramethylbenzidine)
TMB.
• Stop Solution, 1 vial, 6.0 mL of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 mL (40X concentrated),
PBS-Tween
• IFU, 1 copy.
• Plate Lid: 1 piece.
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent
capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with
a precision of better than 1.5%.
• Absorbent paper.
• Distilled water
