T3 ELISA TEST KIT
T3
Triiodothyronine
REF: DS177702 96 tests
1. Intended use
Immunoassay for the in vitro quantitative determination of total
triiodothyronine in human serum.
2. Summary
- Triiodothyronine (T3) is the hormone principally responsible for
the development of the effects of the thyroid hormones on the
various target organs. T3 (3,5,3’-triiodothyronine) is mainly
formed extrathyroidally, particularly in the liver, by enzymatic
5’-deiodination of T4.
- Accordingly, the T3 concentration in serum is more a reflection of
the functional state of the peripheral tissue than the secretory
performance of the thyroid gland.A reduction in the conversion of
T4 to T3 results in a decrease in the T3 concentration.
- It occurs under the influence of medicaments such as propanolol,
glucocorticoids or amiodarone and in severe non-thyroidal illness,
and is referred to as “low T3 syndrome”. As with T4, over 99 % of
T3 is bound to transport proteins.
- However, the affinity of T3 to them is around 10-fold lower.(1, 2,
3, 4)The determination of T3 is utilized in the diagnosis of
T3-hyperthyroidism, the detection of early stages of
hyperthyroidism and for indicating a diagnosis of thyrotoxicosis
factitia.(5, 6, 7)The T3 assay employs a competitive test principle
with polyclonal antibodies specifically directed against T3.
Endogenous T3, released by the action of 8-anilino-1-naphthalene
sulfonic acid (ANS).
2. Reagents Materials provided
• Coated Microplate, 8 x 12 strips, 96 wells, pre-coated with T3
derivant.
• Calibrators, 6 vials, 1 ml each, ready to use; Concentrations:
0(A), 0.5(B), 1(C), 2(D), 4(E) and 8(F) ng/ml.
• Enzyme Conjugate, 1 vial, 6.0 mL of HRP (horseradish peroxidase)
labeled sheep monoclonal Anti-T3 in Tris-NaCl buffer containing BSA
(bovine serum albumin). Contains 0.2% ProClin300 preservative. ANS
1mg/mL.
• Substrate, 1 vial, 11ml, ready to use, (tetramethylbenzidine)
TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/l sulfuric acid.
• Wash Solution Concentrate, 1 vial, 25 ml (40X concentrated),
PBS-Tween wash solution.
• IFU, 1 copy.
• Plate Lid: 1 piece.
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent
capability.=
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with
a precision of better than 1.5%.
• Absorbent paper.
• Distilled water
