Folate
Folic Acid
REF: DS167705 96 tests
Intended use
The Folate ELISA kit is an enzymeimmunoassay intended for the
quantitative determination of Folate in human serum, plasma, tissue
homogenates and other biological fluids.
Summary
Folate, distinct forms of which are known as folic acid, folacin,
and vitamin B9,(1) is one of the B vitamins.(2)The recommended
daily intake of folate in the US is 400 micrograms from foods or
dietary supplements.(3) Folate in the form of folic acid is used to
treat anemia caused by folic acid deficiency.(2) Folic acid is also
used as a supplement by women during pregnancy to prevent neural
tube defects (NTD) in the baby.(2,4)Low levels in early pregnancy
are believed to be the cause of more than half of babies born with
neural tube defects.(5) More than 50 countries use fortification of
certain foods with folic acid as a measure to decrease the rate of
NTDs in the population.(6,7) Long term supplementation is also
associated with small reductions in the risk of stroke and
cardiovascular disease.
(8) It may be taken by mouth or by injection.(2)
Reagents
Materials provided
• Coated Microplate, 96 wells microplate coated with streptavidin.
• Calibrators, 6 vials, 1.0 mL each, ready to use; Concentrations:
0(A), 1.0(B), 2.5(C), 5.0(D), 10(E) and 25(F) ng/mL.
• Folate Enzyme Reagent: 1 vial, 6.0 mL. Contains Folate
(Analog)-horseradish peroxides (HRP) conjugate in a
protein-stabilizing matrix with dye.
• Folate Biotin Reagent: 1 vial, 6.0 mL. Contains biotinylated
purified folate binding protein conjugate in buffer, dye and
preservative.
• Releasing Agent: 1 vial, 12 mL. Contains a strong base (sodium
hydroxide) and potassium cyanide.
• Stabilizing Agent: 1 vial, 6 mL. Contains tris (2-carboxyethyl)
phosphine (TCEP) solution.
• Neutralizing Buffer: 1 vial, 10 mL. Contains buffer with dye that
reduces the pH of sample extraction.
• TMB Substrate, 1 vial, 11 mL, ready to use,
(tetramethylbenzidine) TMB.
• Stop Solution, 1 vial, 6.0 ml of 1 mol/L sulfuric acid.
• Wash Solution Concentrate, 1 vial, 30 mL (20X concentrated),
PBS-Tween wash solution.
• IFU, 1 copy
• Plate Lid, 1 piece
• Sealed Bag, 1 piece
Materials required (but not provided)
• Microplate reader with 450nm and 620nm wavelength absorbent
capability.
• Microplate washer.
• Incubator.
• Plate shaker.
• Micropipettes and multichannel micropipettes delivering 50μl with
a precision of better than 1.5%.
• Absorbent paper.
• Distilled water
