Bovine Brucella Antigen Test With Qualitative And
Preliminary,VECHEK VETERINARY Bovine Range
Application:
The Bovine Brucella Antigen Rapid Test Cassette is a lateral flow
immunochromatographic assay for the qualitative detection of Bovine
Brucella Antigen (B.Brucella Ag ) in cattles serum, plasma, or
whole blood .
| Principle: | Chromatographic Immunoassay |
| Specimen: | WB/S/P |
| Pack: | 10 T |
| Shelf Life: | 2 Years |
| Specificity: | 97.70% |
| Format: | Cassette |
| Reading Time: | 10 Minutes |
| Storage Temperature: | 4-30℃ |
| Sensitivity: | 97.70% |
| Accuracy: | 97.00% |
Description:
The Bovine Brucella Antigen Rapid Test is based on sandwich lateral
flow immunochromatographic assay. The test cassette has a testing
window for the observation of assay running and result reading. The
testing window has an invisible T (test) zone and a C (control)
zone before running the assay. When the treated sample was applied
into the sample well on the cassette, the liquid will laterally
flow through the surface of the test strip and react with the
pre-coated Brucella antibody. If there are anti-Brucella antigens
in the specimen, a visible T line will appears. The C line should
always appear after a sample is applied, which indicates a valid
result. By this means, the cassette can accurately indicate the
presence of Bovine Brucella antigens in the specimen.
How to use:
- Collect fresh whole blood, or separate serum or plasma from blood
as soon as possible to avoid hemolysis. Only use clear,
non-hemolyzed specimens. Do not leave the specimens at room
temperature for prolonged periods. Serum and plasma specimens may
be stored at 2-8 °C for up to 3 days. For long term storage, serum
and plasma specimens should be kept below -20 °C.
- Place the test cassette on a clean and level surface.
For serum and Plasma: Hold the dropper vertically, draw the specimen about 1cm above the
Fill Line, and transfer 2 drops of serum or plasma (approximately 40μl) to the specimen well (S), then add 2 drops of buffer (approximately 120 μl) into the buffer well (B). See illustration
below and start the timer.
For whole blood specimen: Hold the dropper vertically, draw the specimen about 1cm above the
Fill Line, and transfer 1 drop of whole blood (approximately 20μl) to the specimen well (S), then add 2 drops of buffer (approximately 120 μl) into the buffer well (B). See illustration
below and start the timer.
- Read the result at 10 minutes. Do not interpret results after 20 minutes.
