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High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit

    Buy cheap High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit from wholesalers
     
    Buy cheap High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit from wholesalers
    • Buy cheap High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit from wholesalers

    High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit

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    Brand Name : BT Lab
    Model Number : Cat.No E0057Ca
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Payment Terms : Western Union, T/T
    Supply Ability : In Stock
    Delivery Time : 1-3 business days, bulk order within one week
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    High Sensitive Canine Progesterone ELISA Kit , Lab ELISA Sandwich Assay Kit

    Canine Progesterone ELISA Kit with High Spesificity and Sensitivity

    Cat.No E0057Ca

    Standard Curve Range: 1ng/ml - 100ng/ml

    Sensitivity: 0.51ng/ml

    Size: 96 wells

    Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

    * This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.


    Intended Use

    This sandwich kit is for the accurate quantitative detection of Canine Progesterone (also known as PROG) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine PROG antibody. PROG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine PROG Antibody is added and binds to PROG in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PROG antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine PROG. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity

    Standard Solution (128ng/ml)

    0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Canine PROG Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Specimen Collection

    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


    Reagent Preparation

    All reagents should be brought to room temperature before use.

    Standard Reconstitute the 120μl of the standard (128ng/ml) with 120μl of standard diluent to generate a 64ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (64ng/ml) 1:2 with standard diluent to produce 32ng/ml, 16ng/ml, 8ng/ml and 4ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:


    64ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    32ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    16ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    8ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    4ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    128ng/ml64ng/ml32ng/ml16ng/ml8ng/ml4ng/ml

    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


    References

    [1] "High-level expression in Escherichia coli of biologically active bovine growth hormone."George H.J., L'Italien J.J., Pilacinski W.P., Glassman D.L., Krzyzek R.A.DNA 4:273-281(1985)


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