Liquid Biopsy-Specific EDTA Blood Tube with Enhanced Cell Protection and Plastic Cap

IV. Precautions

1. Storage Requirements
2. Store in a dry, cool environment at 2–25℃; avoid direct sunlight, high temperature (above 30℃) and humidity (relative humidity >80%) to prevent degradation of the cell protection formula and anticoagulant.
3. Do not freeze the product. Freezing may cause tube breakage or damage to the inner coating, affecting sample stability.
4. Keep the tubes in their original packaging until use to avoid contamination and light exposure.

6. V. Frequently Asked Questions (FAQs)

7. Microclots observed post-collection. Solution?
   Cause: Inadequate/untimely mixing (especially at >25°C) or underfilled tube.
   Action: Invert ≥8 times; ensure precise fill volume.

8. EDTA-K₂ vs. EDTA-K₃: Key differences?

   • Form: K₂ = crystals; K₃ = liquid spray (faster dissolution).

   • Impact: K₃ overuse dilutes samples (falsely lowers Hb); K₂ offers better batch consistency.

9. Suitable for coagulation tests?
   No! EDTA chelates calcium, disrupting coagulation cascades. Use dedicated citrate tubes (blue cap) instead.

10. Causes/prevention of hemolysis?
    Causes: Blood impacting tube bottom, violent mixing, premature refrigeration (<30 min post-draw).
    Prevention: Angle tubes to let blood flow along walls; use large-bore needles; invert gently.

11. Preventing blood reflux infection?
    Procedure: After draw → Remove tube first → Release tourniquet → Withdraw needle. Prevents negative-pressure backflow.