Introduction of HPLC Liquid Chromatography Instrument:
High-performance liquid chromatography (HPLC) is a powerful analytical technique that separates, identifies and quantifies components in a liquid mixture. Compounds introduced into a solvent pass steadily through a column, and separate within the column as they pass through the packing materials and exit into a detector. The detector tracks and identifies the elements as they exit the column, and a chromatogram chart provides a visual analysis of the compounds.
Technical Parameters of HPLC Liquid Chromatography Instrument LC3400:
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Product Name
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LC3400 Liquid Chromatography Instrument
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Maximum Operating Pressure
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62MPa/75MPa
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Flow Rate Setting Range
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0.0000～12.0000mL/min
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Flow Rate Setting Increment
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0.0001mL/min
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Gradient Error
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≤ 0.75%
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Gradient Stability
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0.2% RSD (5% to 95%, 0.2 to 2.0 mL/min)
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Flow Rate Setting Value Error
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±0.20%
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Flow Rate Stability
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≤0.075% RSD
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Autosampler
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Injection Volume
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100μL, 200μL, 500μL, 1000μL, 1500μL, 2000 μL
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Repeatability
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＜0.3%RSD (2μL～Max),
＜1.0%RSD (0.1μL～1.9μL);
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Linear Correlation Coefficient
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>0.9999
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Number of Sample Position
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108 (2*54, 2mL), 96-well plates
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Temperature Setting Range
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4.0～40.0℃ (optional refrigeration)
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Temperature Setting Value Error
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±0.5℃ (optional refrigeration);
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Temperature Stability
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±1℃ (optional refrigeration)
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Column Oven
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Temperature Setting Range
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4℃-90℃
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Temperature Stability
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±0.03℃/h
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Temperature Setting Value Error
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±0.1℃
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UV Detector
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Baseline Noise
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≤8.0×10-6 Au (1mL/min, methanol, C18)
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Baseline Drift
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≤1.0×10-4 AU/h (1mL/min, methanol, C18)
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Minimum Test Concentration
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≤5×10-9 g/mL (naphthalene/methanol)
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Spectral Range
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188-900nm
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Wavelength Error
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≤ ±1nm
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Wavelength Repeatability
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≤ ±0.1nm
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Linear Range
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≥1×104 or 2.5Au
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Features of HPLC Liquid Chromatography Instrument:
High Pressure Constant Current Pump Features:
1. Small pressure pulsation: the new tandem cam design, integrated with the fifth generation of intelligent algorithms, through the "electronic pulsation suppression" control, reducing the pressure pulsation; further reducing the pressure fluctuations, to ensure that the test results of higher repeatability.
Autosampler
1. Needle in the flow path: Needle in the flow path design, to meet the sample injection zero loss;
2. Customizable cleaning fluids: Customizable cleaning solvents for flexibility in complex application scenarios, combined with internal needle rinsing for extremely low cross contamination.
Heating and Cooling Column Chamber (CT3400)
1. Dual-mode heating: heating-cooling dual-mode temperature control. Standard with semiconductor cooling module, resistance heating module.
2. Air bath: forced air bath circulation temperature control.
3. Temperature setting range: 4℃-90℃;
