Introduction of HPLC Liquid Chromatography Instrument:
HPLC is an abbreviation for High Performance Liquid Chromatography. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. The high performance liquid chromatography instrument can separate and detect each compound by the difference of each compound's speed through the column.
Technical Parameters of HPLC Liquid Chromatography Instrument:
| Product Name | LC3400 Liquid Chromatography Instrument |
| Maximum Operating Pressure | 62MPa/75MPa |
| Flow Rate Setting Range | 0.0000～12.0000mL/min |
| Flow Rate Setting Increment | 0.0001mL/min |
| Gradient Error | ≤ 0.75% |
| Gradient Stability | 0.2% RSD (5% to 95%, 0.2 to 2.0 mL/min) |
| Flow Rate Setting Value Error | ±0.20% |
| Flow Rate Stability | ≤0.075% RSD |
| Autosampler | |
| Injection Volume | 100μL, 200μL, 500μL, 1000μL, 1500μL, 2000 μL |
| Repeatability | ＜0.3%RSD (2μL～Max), ＜1.0%RSD (0.1μL～1.9μL); |
| Linear Correlation Coefficient | >0.9999 |
| Number of Sample Position | 108 (2*54, 2mL), 96-well plates |
| Temperature Setting Range | 4.0～40.0℃ (optional refrigeration) |
| Temperature Setting Value Error | ±0.5℃ (optional refrigeration); |
| Temperature Stability | ±1℃ (optional refrigeration) |
| Column Oven | |
| Temperature Setting Range | 4℃-90℃ |
| Temperature Stability | ±0.03℃/h |
| Temperature Setting Value Error | ±0.1℃ |
| UV Detector | |
| Baseline Noise | ≤8.0×10-6 Au (1mL/min, methanol, C18) |
| Baseline Drift | ≤1.0×10-4 AU/h (1mL/min, methanol, C18) |
| Minimum Test Concentration | ≤5×10-9 g/mL (naphthalene/methanol) |
| Spectral Range | 188-900nm |
| Wavelength Error | ≤ ±1nm |
| Wavelength Repeatability | ≤ ±0.1nm |
| Linear Range | ≥1×104 or 2.5Au |
Features of HPLC Liquid Chromatography Instrument:
High Pressure Constant Current Pump Features:
1. Small pressure pulsation: the new tandem cam design, integrated with the fifth generation of intelligent algorithms, through the "electronic pulsation suppression" control, reducing the pressure pulsation; further reducing the pressure fluctuations, to ensure that the test results of higher repeatability.
2. Acetonitrile Resistant Check Valve: Adopting a new type of check valve greatly reduces the possible failure of the check valve when pure acetonitrile is used in the system.
3. Liquid leakage detection: Used for alarming in case of liquid leakage caused by misoperation, aging of pipes, etc;
4. Mixer: Titanium alloy mixer, gradient mixing more uniform.
5. Active flushing of seals greatly improves seal and plunger rod life.
Vacuum Membrane Degassing System:
1. Built-in 5-channel vacuum pump, pressure sensor, real-time monitoring of vacuum cavity pressure changes, to ensure timely and efficient degassing operation.
2. Vacuum maintenance: automatically monitor the vacuum status, vacuum decreases, automatically start the vacuum pump, effectively maintain the vacuum degree.
