TB Stain
(Fluorescence Method)
Intended use:
For identification of acid-fast bacteria of the genus Mycobacterium, which includes the tuberculosis-causing Mycobacterium tuberculosis
Principle:
When the acid-fast bacteria are stained by fluorescent dye Auramine O, bright orange-yellow fluorescence can be easily detected by UV-fluorescence microscope under low magnification. It is faster to screen acid-fast bacteria using this fluorescence kit than the Carbolfuchsin stain.
Methods:
1. Fix smear, then add Solution A to stain for 15 minutes at room temperature or under heat for 5 minutes. Rinse with water gently.
2. Decolorize for 1~2 minutes with Solution B. Rinse with water gently.
3. Counterstain with Solution C for 2~4 minutes. If counterstain for too long, the fluorescence will disappear. Rinse with water gently.
4. Air dry and then examine the finished smear in the black background.
Specifications:
Contents | 3Btlsx250ml/kit | Components |
Auramine O dye | 1BTx250ml | Auramine O |
Acid Alcohol | 1BTx250ml | 0.5% hydrochloric acid ethanol |
Counterstain solution | 1BTx250ml | 0.5% potassium permanganate |
Precaution:
1. All positive smears should be restained by Kinyoun and Ziehl-Neelsen methods for confirmation.
2. The Auramine O dye included in this kit is very stable and will not precipitate. As a result, the fluorescence would not disappear.
Expected Results:
The acid-fast mycobacteria appear bright orange yellow in black background, while the debris in the background are in light yellow.
