Melamine ELISA Test Kit
Product Description
REAGEN™ Melamine ELISA Test Kit is a competitive enzyme immunoassay for
the quantitative analysis of melamine in milk, yogurt, sour milk,
milk powder, egg, meat, fish, feedand other food samples.
The unique features of the kit
1. High recovery (>80%),rapidand cost-effective extraction
methods.
2. High sensitivity(5ng/g or ppb)and low detection limit for liquid
milk (25ppb).
3. A quick ELISA assay(less than 1 hours regardless of number of
samples).
4. High reproducibility.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
drug of interest has been coated in the plate wells. During the
analysis, sample and HRP-Conjugated Antibody #2 are added along
with the primary antibody specific for the target drug. If the
target is present in the sample, it will compete for the antibody,
thereby preventing the antibody from binding to the drug attached
to the well. At the same time, the second Antibody, tagged with a
peroxidase enzyme, targets the primary antibody that is complexed
to the drug coated on the plate wells. The resulting color
intensity, after addition of substrate, has an inverse relationship
with the target concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Milk | 0.025 |
| Yogurt/sour milk | 0.025 |
| Milk powder/Infant Formula | 0.2 |
| Meat /fish/Egg | 0.125 |
| Feed | 0.25 |
| Milk | 0.025 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Melamine | 100 |
| Cyanurate | 15 |
| Ammeline | 5 |
| Ammelide | <1 |
