Sulfamethoxazole ELISA Test Kit
Product Description
REAGEN™ Sulfamethoxazole ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Sulfamethoxazole in
feed, fish, eggs, honey, meat (beef, chicken and pork), milk, milk
powder, serum, shrimp and urine.
The unique features of the kit are:
1.Rapid extraction method for various samples with high recovery
(80-105%).
2.High sensitivity (0.5 ppb) and low detection limit (0.25 ppb for
shrimp).
3.High reproducibility.
4.A quick ELISA assay (less than 2 hours regardless of number of
samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
plate wells are coated with capture antibodies directed against
anti- Sulfamethoxazole antibodies. Sulfamethoxazole standards or
sample solution, Sulfamethoxazole–HRP conjugate and
anti-Sulfamethoxazole antibodies are added. Free Sulfamethoxazole
and Sulfamethoxazole-HRP Conjugate compete for the Sulfamethoxazole
antibody binding sites. At the same time, the anti-
Sulfamethoxazole antibodies are also bound by the immobilized
capture antibodies. Any unbound Sulfamethoxazole-HRP Conjugate is
then removed in a washing step. The TMB Substrate is added to the
wells. The resulting color intensity, after addition of substrate,
has an inverse relationship with the target concentration in the
sample.
Kit Contents, Storage and Shelf Life
REAGEN™ Sulfamethoxazole ELISA Test Kit has the capacity for 96
determinations of 42 samples in duplicate (assuming 12 wells for
standards). Return any unused microwells to the foil bag and reseal
them with the desiccant provided in the original package. Store the
kit at 2-8°C *. The shelf life is 12 months when the kit is
properly stored.
