Tetracycline(TET) ELISA Test Kit
Product Description
REAGEN™ Tetracycline (TET) ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Tetracycline in in
honey, butter, whey, egg, cheese, milk, meat, meat products such as
sausage, fish and shrimp.
The unique features of the kit are:
1. High recovery (75-120%), rapid (less than 30 minutes), and
cost-effective extraction methods.
2. High sensitivity (0.05 ng/g or ppb) and low detection limit for
samples.
3. High reproducibility.
4. A quick ELISA assay (less than 2 hours regardless of number of
samples).
Procedure Overview
REAGEN™ Tetracycline (TET) ELISA Test Kit is based on a competitive
colorimetric ELISA assay. The drug of interest has been coated in
the plate wells. During the analysis, sample is added along with
the primary antibody specific for the target drug. If the target is
present in the sample, it will compete for the antibody, thereby
preventing the antibody from binding to the drug attached to the
well. The secondary antibody, tagged with a peroxidase enzyme,
targets the primary antibody that is complexed to the drug coated
on the plate wells. The resulting color intensity, after addition
of substrate, has an inverse relationship with the target
concentration in the sample.
Sensitivity (Detection Limit)
Sample Type | Detection Limit (ng/g or ppb) |
Egg/Cheese/Sour Cream | 1.5 |
Honey | 3 |
Meat/Meat ProductsFish/Shrimp/Butter | 0.5 |
Milk/Soured Milk/Yogurt/Curd | 0.5 |
Reconstituted Milk Powder | 1.5 |
Reconstituted Whey Powder | 1.5 |
Feed | 8 |
Specificity (Cross-Reactivity)
Analytes | Cross-Reactivity (%) |
Tetracycline (TET) | 100.0 |
Chlortetracycline | 124 |
Oxytetracycline | 40 |
Doxycycline | 25 |
Reqired Materials Not Provided With the Kit
1. Microtiter plate reader (450 nm)
2. Vortex mixer, (e.g. Gneie Vortex mixer from VWR)
3. 10, 20, 100 and 1000 mL pipettes
4. Multi-channel pipette: 50-300 mL (Optional)
Warnings and Precautions
1. The standards contain tetracycline. Handle with particular care.
2. Do not use the kit past the expiration date.
3. Do not intermix reagents from different kits or lots except for
components with the same part No’s within their expiration dates.
ANTIBODIES AND PLATES ARE KIT-AND LOT-SPECIFIC.
4. Try to maintain a laboratory temperature of 20–25°C (68°–77°F).
Avoid running assays under or near air vents, as this may cause
excessive cooling, heating and/or evaporation. Also, do not run
assays in direct sunlight, as this may cause excessive heat and
evaporation. Cold bench tops should be avoided by placing several
layers of paper towel or some other insulation material under the
assay plates during incubation.
5. Make sure you are using only distilled or deionized water since
water quality is very important.
6. When pipetting samples or reagents into an empty microtiter
plate, place the pipette tips in the lower corner of the well,
making contact with the plastic.
7. Incubations of assay plates should be timed as precisely as
possible. Be consistent when adding standards to the assay plate.
Add your standards first and then your samples.
8. Add standards to plate only in the order from low concentration
to high concentration as this will minimize the risk of
compromising the standard curve.
9. Always refrigerate plates in sealed bags with a desiccant to
maintain stability. Prevent condensation from forming on plates by
allowing them equilibrate to room temperature (20 – 25℃ / 68 – 77℉)
while in the packaging.
