Nicarbazin ELISA Test Kit , Feed , Chicken , Pork , Egg and Beef , high recovery rate of 75 - 95%

Nicarbazin ELISA Test Kit

Product Description

REAGEN™ Nicarbazin ELISA Test Kit enables government agencies, food manufacturers and quality assurance organizations to detect nicarbazin in Feed,Chicken,Pork,Egg and Beef to satisfy customer concerns about food safety.

The unique features of the kit are:

1. The extraction of nicarbazin from samples can be finished in 10 - 30 minutes with a high recovery rate of 75 - 95%.

2. A quick ELISA assay (less than 1.5 hours regardless of number of samples).

3. High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The nicarbazin antibody has been coated in the plate wells. During the analysis, sample is added along with the Nicarbazin-horseradish peroxidase conjugate. If the nicarbazin residue is present in the sample, it will compete for the nicarbazin antibody, thereby preventing the nicarbazin-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the nicarbazin residue concentration in the sample.

Feed

• Homogenize a reasonable amount of sample with a suitable mixer.
• Weigh out 1g of the homogenized sample and mix with 5 mL of acetonitrile.
• Vortex for 1 minute at maximum speed.
• Centrifuge for 10 minutes at 4,000 x g at room temperature (20 – 25°C / 68 – 77°F).
• Transfer 20 µL of the supernatant to a 2 mL-tube ,add 980µL 1X Sample Diluent mix well.
• Use 100 µL in the assay.

Note: Dilution factor: 250.