Salbutamol ELISA Test Kit
Product Description
REAGEN™ Salbutamol ELISA Test Kit is a competitive enzyme immunoassay for
the quantitative analysis of Salbutamol in feed, fish, honey, meat
(beef, chicken and pork), milk, milk powder, serum, and urine.
The unique features of the kit are:
1. Rapid (10 - 30 minutes) extraction method for various samples
with high recovery (75 - 115%).
2. High sensitivity (0.05 ng/g or ppb) and low detection limit
(0.025 ng/g or ppb for meat/tissue, 0.25 ng/g or ppb for urine and
1 ng/g or ppb for animal feed).
3. High reproducibility.
4. A quick ELISA assay.
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
drug of interest has been coated in the plate wells. During the
analysis, sample is added along with the primary antibody specific
for the target drug. If the target is present in the sample, it
will compete for the antibody, thereby preventing the antibody from
binding to the drug attached to the well. The secondary antibody,
tagged with a peroxidase enzyme, targets the primary antibody that
is complexed to the drug coated on the plate wells. The resulting
color intensity, after addition of substrate, has an inverse
relationship with the target concentration in the sample.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Feed | 1 |
| Honey | 1 |
| Meat/Liver | 0.1 |
| Swine/Bovine Liver and Kidney | 0.1 |
| Milk/serum | 0.25 |
| Urine | 0.05method1;0.25method 2 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Salbutamol | 100 |
| Salbutamol sulfate | 100 |
| Clenbuterol | 60 |
| Mapenterol | 12 |
| Tolubuterol | 6.8 |
| Terbutaline | 1.4 |
| Cimbuterol | 0.7 |
| Mabuterol | 0.5 |
| Cimaterol | 0.1 |
| Pirbuterol | <0.1 |
| Isoprenaline | <0.1 |
