Cefoperazone ELISA Test Kit
Product Description
REAGEN™ Cefoperazone ELISA Test Kit is a competitive enzyme
immunoassay for the quantitative analysis of Cefoperazone in milk,
urine, tissue (liver, kidney, and muscle), honey, serum, swipe
sample and plasma samples.
The unique features of the kit are:
1. High recovery (>75%), low-cost rapid sample prep protocol (10
minutes - 1 hour).
2. High sensitivity: 0.25 ng/gram = 0.25 ppb (parts per billion).
3. Rapid assay protocol (less than 2 hours to process 40 samples in
duplicate).
4. High reproducibility (interplate and intraplate CV typically
<10%).
Procedure Overview
REAGEN™ The method is based on a competitive colorimetric ELISA
assay. The drug of interest (Cefoperazone) has been coated in the
plate wells. The sample to be tested is mixed with a high-affinity
Cefoperazone capture protein and added to the well. If the
Cefoperazone target is present in the sample, it will compete for
the capture protein, thereby preventing the capture protein from
binding to the drug attached to the well. The secondary antibody,
tagged with a peroxidase enzyme, targets the capture protein bound
to the drug coated on the plate wells. The resulting color
intensity, after addition of substrate, has an inverse relationship
with the target concentration in the sample. The levels of
Cefoperazone in the sample are determined by comparison of the
color intensity of unknown samples with the standard curve, which
is made using the Cefoperazone standard provided in the kit.
Sensitivity (Detection Limit)
| Sample Type | Detection Limit (ng/g or ppb) |
| Meat, Tissue, Fish, Swipe Sample and Urine | 2.5 |
| Serum and Plasma | 5 |
| Honey | 6.25 |
| Milk | 12.5 |
Specificity (Cross-Reactivity)
| Analytes | Cross-Reactivity (%) |
| Cefoperazone | 100 |
| Amoxicillin | 129 |
| Cloxacillin | 117 |
| Oxacillin | 117 |
| Ceftiofur | 74 |
