Research Use Vitamin Test Kit / Rapid Vitamin B12 ELISA Test Kit

Vitamin B12 ELISATest Kit

Product Description

REAGEN^™ Vitamin B12 Test Kit is quantitative analysis of total Vitamin B12 in food ,feed and drug.

The unique features of the kit are:

• rapid, and cost-effective extraction methods,the same as other Vitamin
• The kit provide positive control and serial standards
• High sensitivity (0.06μg/100g)
• High recovery(90%-105%)
• High reproducibility

Procedure Overview

REAGEN^™Sample and Vitamin B12 culture medium add to the 96-microwell which has the Lactobacillus delbrueckii subsp. lactis (leichmannii),if the sample has vitamin B12 the Lactobacillus delbrueckii will grow,after culture 37℃,44-48h, read the OD by microplate reader 610 - 630 nm (or540 - 550 nm),The sample of the turbidity compared with standard curve can get Vitamin B12 content.

Kit Contents, Storage and Shelf Life

^REAGEN™ Vitamin B12 Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8℃ .The shelf life is 12 months when the kit is properly stored.