Vitamin B5 (Pantothenic acid) Test Kit
Product Description
REAGEN™Vitamin B5 Test Kit is quantitative analysis of total Vitamin B5 in
food ,feed and drug.
The unique features of the kit are:
1. rapid, and cost-effective extraction methods,the same as other
Vitamin.
2. The kit provide positive control and serial standards
3. High sensitivity (80μg/100g)
4. High recovery(80%-120%)
5. High reproducibility
Procedure Overview
REAGEN™Sample and Vitamin B5 culture medium add to the 96-microwell which
has the Lactobacillus plantarum, if the sample has Vitamin B5 the
Lactobacillus plantarum will grow, after culture 37℃,44-48h, read
the OD by microplate reader 610 - 630 nm (or540 - 550 nm),The
sample of the turbidity compared with standard curve can get
Vitamin B5 content.
Test protocol
All the reagents need to return to room temperature.shake the
reagents before use. Don't pollute the kit’s reagents.
Add 2.5 mL sterile water to standard , cover cap and shake points
dissolved with standard. The standard concentrate is 2400ug/100g.
Filter the standard to a 4mL tube by 0.2um sterile filter.
