DOA BAR rapid Test with CE certificate urine Uncut sheet

INTENDED USE

The One Step Barbiturates Test is a lateral flow chromatographic immunoassay for the detection of Barbiturates in human urine at the cut-off concentration of 300 ng/ml. This assay provides only a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.

PERFORMANCE CHARACTERISTICS

A. Sensitivity

One Step Barbiturates Test has set the screen cut-off for positive specimens at 300 ng/mL for secobarbital as a calibrator. The test device has been proved to detect above 300 ng/mL of Barbiturates in urine at 5 minutes.

B. Specificity and cross reactivity

To test the specificity of the test, the test device was used to test barbiturates, metabolites and other components of the same class that are likely to be present in urine, All the components were added to drug-free normal human urine. These concentrations below also represent the limits of detection for the specified drugs or metabolites.

Component Concentration (ng/ml)

Secobarbital 300

Amobarbital 300

Alphenol 150

Aprobarbital 200

Butabarbital 75

Butathal 100

Butalbital 2,500

Cyclopentobarbital 600

Pentobarbital 300

Phenobarbital 100

C. Interfering substances

Considering the complexity of clinical urine specimens and the possibility that various urine specimens contain potentially interfering substances, for example Acetoacetic Acid, Acetone, Albumin etc., we simulated above situations by adding the potentially interfering substances to a certain concentration as specimen.

SUMMARY

Barbiturates are a class of central nervous system depressions. They have a wide range of half-life of 2 to 40 hours and can be detected in the urine for 1 to 4 days after use. Phenobarbital is a long acting barbiturate derivative that has been used as a daytime sedative and very extensively as an anticonvulsant. Pentobarbital and secobarbital are two examples of a short acting barbiturate sedative. Abuse of barbiturates can lead not only to impaired motor coordination and mental disorder, but also to respiratory collapse, coma and even death. Barbiturates are taken orally, rectally, or by intravenous and intramuscular injections. Short-acting barbiturates will generally be excreted in urine as metabolites, while the long-acting barbiturates will primarily appear unchanged.