HPLC Cromatografía Fully Automatic High Performance Liquid
Chromatography Instrument
Description of High Performance Liquid Chromatography Instrument
High performance liquid chromatography is basically a highly
improved form of column chromatography. Instead of a solvent being
allowed to drip through a column under gravity, it is forced
through under high pressures of up to 400 atmospheres. That makes
it much faster.
It also allows you to use a very much smaller particle size for the
column packing material which gives a much greater surface area for
interactions between the stationary phase and the molecules flowing
past it. This allows a much better separation of the components of
the mixture.
The other major improvement over column chromatography concerns the
detection methods which can be used. These methods are highly
automated and extremely sensitive.
Technical Data of High Performance Liquid Chromatography Instrument
| Manufacturer: | Wayeal |
| Product Name: | Liquid Chromatography Instrument |
| Working Power Supply | 220V, 50Hz |
| Ambient Temperature | 10~40°C |
| Relative Humidity | 20~85% |
| Qualitative Repeatability | ≤0.2% |
| Quantitative Repeatability | ≤0.4% |
| High Pressure Constant Flow Pump | |
| Flow Rate Range | 0.001~10.000mL/min |
| Setting Step | 0.001mL/min |
| Pressure Range | 0~45MPa |
| Pressure Pulsation | 0.1MPa |
| Flow Rate Set Error | ±0.2% |
| Flow Rate Stability | RSD≤0.06% |
| Gradient Error | ±1% |
| Column Oven | |
| Temperature Control Range | 4~85°C |
| Temperature Stability | ±0.02°C |
| Temperature Setting Resolution | 0.1°C |
| Autosampler | |
| Injection Volume Repeatability | ＜0.5%RSD |
| Linearity | ＞0.9999 |
| Cross Contamination | ＜0.002% |
| Injection Volume Accuracy Error | ±1% |
| Sample Positions | 120 |
| Injection Volume | 1~100μL |
| UV Detector | |
| Baseline Noise | ≤2.0*10-6Au |
| Baseline Drift | ≤1*10-5Au/h |
| Mini Detection Concentration | ≤3.0*10-9g/mL (naphthalene/methanol) |
| Spectral Range | 188~900nm |
| Wavelength Error | ≤±0.1nm |
| Wavelength Repeatability | ≤±0.1nm |
| Linearity Range | ≥103 |
| DA Detector | |
| Baseline Noise | ≤4*10-5Au |
| Baseline Drift | ≤3*10-5Au/h |
| Mini Detection Concentration | ≤2*10-8g/mL |
| Spectral Range | 190~800nm |
| Wavelength Error | ≤±0.1nm |
| Wavelength Repeatability | ≤±0.1nm |
| Linearity Range | ≥ 104 |
| ELS Detector | |
| Baseline Noise | ≤ 0.0037mV |
| Baseline Drift | ≤ 0.0026mV/30min |
| Evaporation Gas Flow Range | (0 ~ 3) SLM |
| Atomization Temperature Range | Room temperature ~ 90℃ |
| Evaporation Temperature Range | Room temperature～110℃ |
| Detection Chamber Temperature Range | Room temperature～60℃ |
| Input Air Pressure | (4 ~ 7) bar |
| FL Detector | |
| Baseline Noise | ≤ 5×10-6 FU |
| Baseline Drift | ≤ 2 × 10-5 FU/30min |
| Spectral Range | (200 ~ 650) nm |
| Wavelength Error | ± 0.1 nm |
| Linearity Range | ≥ 104 |
| RI Detector | |
| Detection Range | 0.25 ~ 512 μRIU |
| Linearity Range | ≥ 600 μRIU |
| Response Time | 0.1, 0.25, 0.5, 1, 1.5, 2, 3, 6 sec; |
| Cell Volume | 8μL |
Features of High Performance Liquid Chromatography Instrument
1. The HPLC system features a needle-in-flow-path injector,
enabling sample injection without sample loss and minimizing
cross-contamination.
2. It is equipped with online derivatization and online dilution
functions for enhanced analytical flexibility.
3. The system incorporates an optimized and patented needle washing
path design, ensuring cleaning efficiency and reliability.
4. An innovative check valve technology guarantees excellent
solvent resistance, particularly for acetonitrile (ACN).
5. The column oven with integrated valve switching accommodates two
250mm HPLC columns and can be flexibly expanded to accommodate four
UHPLC columns.
5. Powered by the patented H.B.C. algorithm, the system
intelligently identifies shoulder peaks and minor peaks, featuring
a wizard-guided operation mode and intelligent integration for
user-friendly handling.
Applications of High Performance Liquid Chromatography Instrument
Determination of Aflatoxin B and G groups, Nutrients, Additives, Aflatoxin M,
Heterotrophic trichothecene, Penicillin, Erythrostenone,
Deoxynivalenol and its acetylated derivatives, Ochratoxin A, T-2
toxin, Fumonisins in food.
