RUO Antibodies to Herpes Simplex Virus Ⅱ(HSV Ⅱ) IgM ELISA Test Kit
This kit is a qualitative detection of human serum/plasma of HSV Ⅱ
IgM . The kit is suitable for clinical screening and diagnosis of
HSV Ⅱ infection in serum/plasma.
MAIN COMPONENTS
Materials provided with the kit:
| Components | 96T/Box | 480T/Box |
| Coated Microtiter Plate | 1 bag | 8*12 | 5 bags | 8*12 |
| Conjugate | 1 vial | 6.5 mL | 5 vials | 6.5 mL |
| Wash Buffer (40X) | 1 vial | 20 mL | 5 vials | 20 mL |
| Sample Diluent | 1 vial | 11 mL | 5 vials | 11 mL |
| Substrate A | 1 vial | 7 mL | 5 vials | 7 mL |
| Substrate B | 1 vial | 7 mL | 5 vials | 7 mL |
| Stop Solution | 1 vial | 6 mL | 5 vials | 6 mL |
| Negative Control | 1 vial | 1 mL | 5 vials | 1 mL |
| Positive Control | 1 vial | 1 mL | 5 vials | 1 mL |
| Closure Plate Membrane | 3 sheets | | 15 sheets | |
Note: different batches of reagent kit, and different component
cannot be exchanged for use. Once open, stable for 3 months at 2-8°C.
TEST PROCEDURE
1. All reagents should be allowed to reach room temperature for 15
minutes before use.
2. Dilute the wash buffer at the rate of 1:40 dilution with
distilled water before use.
3. Add 100μL Sample Diluent in the corresponding well (Do not add
in the blank well, negative control wells and positive control
well.) The sample should be corresponding to the number of micro
plate, each plate should be provided with negative control 2 wells,
positive control 1 well and blank control 1 well. (If detect with
dual wavelength detection, setting no blank control well is
allowed).
Note: Use a separate disposal pipette tip for each specimen,
Negative and Positive Control to avoid cross contamination.
4. Add 10μL sample in the corresponding well, mix thoroughly by
using the pipette, add 100μL negative control and positive control
to negative control wells and positive control well (Do not add in
the blank well).
5. Shake gently to mix for 30s. Incubate at 37°C for 20 minutes
with the sealing plate membrane sealing the plate.
6. At the end of the incubation, remove and discard the plate
cover. Take out, add wash buffer to each well for 20 seconds.
Repeat 5 times. After the final washing cycle, turn the plate over
onto blotting paper or clean towel, and tap it to remove any
remainders.
7. Respectively adding Conjugate 50μL (Do not add in the blank
well) Incubate at 37°C for 20 minutes with the sealing plate
membrane sealing the plate.
8. Repeat the wash step for 5 times as in step 6.
9. Add Substrate A (50µL) and Substrate B (50µL) (Do not add in the
blank well). Incubate at 37°C for 10 minutes with the sealing plate
membrane sealing the plate.
10. Add 50μL Stop Solution to each well (Do not add in the blank
well). Mix gently by shaking, read the absorbance within 10 minutes
after stopping the reaction. Calibrate the plate reader with the
blank well and read the absorbance at 450nm. If a dual filter
instrument is used, set the reference wavelength at 630nm. Set no
blank well is allowed if use dual wavelength to detect. Calculate
the Cut-Off value and evaluate the results.
