Human Vitamin D ELISA Kit
INTENDED USE
Our Human Vitamin D ELISA kit is to assay Vitamin D levels in Human
serum, plasma, culture media or any biological fluid.
| Product details | Description |
| Delivery | Within 48 hours |
| Packaging Specifications | 8 x 12 strips, 96 wells |
| Country Of Origin | China |
| Manufacturer | 18 months |
| Preservation method | 2℃-8℃ |
| Specimen | Whole blood |
| Assification | class1 |
| Type | Elisa Test Kit |
Sample preparation
1. Serum preparation
After collection of the whole blood, allow the blood to clot by
leaving it undisturbed at room temperature. This usually takes
10-20 minutes. Remove the clot by centrifuging at 2,000-3,000 rpm
for 20 minutes. If precipitates appear during reservation, the
sample should be centrifugated again.
2. Plasma preparation
Collect the whole blood into tubes with anticoagulant (EDTA or
citrate). After incubated at room temperature for 10-20 minutes,
tubes are centrifugated for 20 min at 2,000-3,000 rpm. Collect the
supernatant carefully as plasma samples. If precipitates appear
during reservation, the sample should be centrifugated again.
3. Urine samples
Collect urine into aseptic tubes. Collect the supernatant carefully
after centrifuging for 20 min at 2,000-3,000 rpm. If precipitates
appear during reservation, the sample should be centrifugated
again. The preparation procedure of cerebrospinal fluid and
pleuroperitoneal fluid is the same as that of urine sample.
4. Cell samples
If you want to detect the secretions of cells, collect culture
supernatant into aseptic tubes. Collect the supernatant carefully
after centrifuging for 20 min at 2,000-3,000 rpm. If you want to
detect intracellular components, dilute the cells to 1X100/ml with
PBS (pH 7.2-7.4). The cells were destroyed to release intracellular
components by repeated freezing and thawing. Collect the
supernatant carefully after centrifuging for 20 min at 2,000-3,000
rpm. If precipitates appear during reservation, the sample should
be centrifugated again.
5. Tissue samples
Tissue samples are cut, weighed, frozen in liquid nitrogen and
stored at -80℃ for future use. The tissue samples were homogenized
after adding PBS (pH 7.4). Samples should be operated at 4℃.
Collect the supernatant carefully after centrifuging for 20 min at
2,000-3,000 rpm. Aliquot the supernatant for ELISA assay and future
use.
Materials provided with the kit
Notes:
- Store the kit at 4°C upon receipt.The kit should be equilibrat4ed
to room temperature before the assay. Remove any unneeded strips
from Human Vitamin D Antibody-Coated plate, reseal them in zip-lock
foil and keep at 4°C.
- Precipitates may appear in concentrated washing buffer. Please heat
the buffer to dissolve all the precipitates, which will not affect
the results.
- Accurate pipette should be used to avoid experimental error.
Samples should be added to the Microplate in less than 5 minutes.
If a large number of samples are included, multiple channel pipette
is recommended.
- Standard curve should be included in every assay. Replicate wells
are recommended. If the OD value of the sample is greater than the
first well of standards, please dilute the sample (n times) before
test. When calculating the original Vitamin D concentration, please
multiply the total dilution factor (XnX5).
- In order to avoid cross-contamination, Microplate sealers are for
one-time use only.
- Please keep Substrate away from light.
- All the operation should be accordance with the manufacturer's
instructions strictly. The results determined by the Microtiter
Plate Reader.
- All the samples, washing buffer and wastes should be treated as
infectious agents.
- Reagents from different lots should not be mixed.
Storage and validity
1．Storage: 2-8℃.
2．validity: six months
