Macroscopic observation , Seminal Reactive Oxygen Species NBT Staining Kit

Macroscopic observation

Rapid test for sperm superoxide anion (O₂^-.)

Seminal Reactive Oxygen Species NBT Staining Kit

Applications:

• Analyze the etiology of infertility.
• Useful supplements for routine semen analysis, can be carried out routinely.
• Patient ROS level detection and efficacy monitoring after antioxidant treatment.
• Monitor the change of seminal ROS, and select the optimal sperm for fertilization.
• Analyze the reasons of ART adverse outcome.
• Analyze the reasons of low quality semen parameters

Features:

• The test process is simple, no special equipment required.
• Easy semi-quantitative detection with a small amount of semen.
• Required equipment: ordinary 37 ℃ water bath.

Test process:

Test results:

The NBT test method is adopted in the form of chromogenic reactions, and the chromogenic product is derived

from the formazan, the reductive substance of tetrazole.Semi-quantitative detection of ROS (O₂⁻ •) is based on

the color of formazan.

Diagram of formazan deposit by NBT reaction of Sperm/leukocyte ROS

(a) Negative sperm; (b) Sperm with positive head;

(c) Sperm with positive midpiece; (d) Sperm with positive acrosome;

(e) Weakly positive leukocyte; (f) Strongly positive leukocyte.

BRED Life Science Technology Inc. specializes in developing, manufacturing and marketing lab products in the field of reproductive medicine, offering automatic assay platform and related products for infertility diagnosis and ART treatment. Based on the test methods and standard recommended by the World Health Organization, BRED develops serial products for infertility diagnosis and ART treatment with innovative technology. And these help promote the establishment and popularization of standardization for reproductive medicine lab. BRED own a completest range of relative products in the field of reproductive medicine worldwide.The products are of independent intellectual property right within BRED.

Our mission of development is to be devoted to providing the best solutions and products for the clinical laboratory in reproductive medicine with the aim to promote the development of human reproductive health.

Our operating philosophy is putting people first, doing business on the basis of honesty, forging ahead and working with diligence.

Welcome to visit our official website: www.spermfunc.com