Saxitoxin (PSP) ELISA Test Kit
Product Description
REAGEN™ Saxitoxin (PSP) ELISA Test Kit is a competitive enzyme immunoassay
for the quantitative analysis of Saxitoxin in mussels and water
samples. Saxitoxin, the principal component of paralytic shellfish
poisons (PSP), blocks the sodium ion channels in nerve and muscle
membranes.The lethal dose in humans is 1 to 3 mg. Numbness and
respiratory paralysis can occur after oral ingestion of as little
as 0.5 - 1.0mg saxitoxin. Saxitoxin residues can be determined by
either GC-MS or HPLC. However, enzyme immunoassays (ELISA) show
considerable advantages as it is faster and more sensitive.
REAGEN™ Saxitoxin (PSP) ELISA Test Kit enables international and
government regulatory agencies, food manufacturers and processors,
as well as quality assurance organizations, to detect saxitoxin in
shellfish matrices and to satisfy customer concerns about food
safety.
The unique features of the kit are:
1. High recovery (80%-105%), rapid (10 - 30 minutes), and
cost-effective extraction methods
2. High sensitivity (0.05 ng/g or ppb) and low detection limit (3
ppb for mussel samples and 1 ppb for water samples).
3. High reproducibility.
4. A quick ELISA assay(less than 1.5 hours regardless of number of
samples).
Procedure Overview
The method is based on a competitive colorimetric ELISA assay. The
second antibody against anti-saxitoxin antibody was coated in the
plate wells. During the analysis, sample is added along with the
saxitoxin-horseradish peroxidase (Saxitoxin-HRP) conjugate and
anti-saxitoxin antibody. If the saxitoxin residue is present in the
sample, it will compete with Saxitoxin-HRP for the anti-saxitoxin
antibody, thereby preventing the Saxitoxin-HRP from binding to the
anti-Saxitoxin antibody. The anti-saxitoxin antibodies are then
bound by the second antibody immobilized on the plate. After a
washing step and addition of the TMB substrate solution, a color
signal is produced. The color reaction is stopped after a specified
time and the color is evaluated using an ELISA reader. The
resulting color intensity has an inverse relationship with the
saxitoxin residue concentration in the sample.
Sensitivity (Detection Limit)
Sample Type | Detection Limit (ng/g or ppb) |
Mussels | 3 |
Water | 1 |
Specificity (Cross-Reactivity)
Analytes | Cross-Reactivity (%) |
Saxitoxin | 100 |
Neosaxitoxin | 20 |
Decarbamoyl Saxitoxin | 100 |
GTX 2 & 3 | 43 |
GTX 1 & 4 | 2 |
Decarbamoyl GTX 2 & 3 | 10 |
Decarbamoyl Neosaxitoxin | 4 |
GTX-5 | 61 |
