Tetrodotoxin(TTX) ELISA Test Kit
Product Description
Tetrodotoxin (TTX) is a powerful neurotoxin, which is tolerance to
heat, salt and cooking .Minimum lethal dose for human is about
0.5mg/60 kg of body weight, The toxicity is 1000 times great than
the sodium cyanide. Every year many people are ill due to improper
eating or eating puffer fish. Therefore, it is significance to
accurate detection of tetrodotoxin in puffer fish in order to
prevention and control of tetrodotoxin poisoning . The detection
method is sensitive, fast, simple and specific, only need a small
amount of fish sampling.
Procedure Overview
The REAGEN™ Tetrodotoxin indirect competitive ELISA is an immunoassay for the
quantitative and sensitive detection of Tetrodotoxin in water
samples and puffer fish samples . Antigen is coated in test wells
,The sample extract and calibrators are pipetted into the test
wells followed by TTX monoclone antibody into the test wells to
initiate the reaction. After this 90 minutes incubation the wells
are washed with wash solution. Then add enzymes labeled anti-mouse
IgG antibody, incubation, then adding the color solution, terminate
the reaction by the stop solutions, measured the amount of color in
each well with a microplate reader at OD 450nm The color of the
unknown samples is compared to the color of the calibrators and the
TTX concentration of the samples is derived.
Kit Contents, Storage and Shelf Life
The REAGEN™ Tetrodotoxin ELISA kit in its original packaging can be used until
the end of the month indicated on the box label when stored at 2-
8ºC.
1. Plate containing 12 test strips of 8 wells each vacuum-packed in
aluminized pouch with indicating dessicant.
2. 5 vials each containing 0.6 mL of TTX calibrators corresponding
to 0, 10, 30, 90 and 270 ppb.
3. 1 vial containing 6 mL of TTX antibody Solution.
4. 1 vial containing 11 mL of enzyme conjugate Solution,
5. 1 vial containing 11 mL of colour solution.
6. 1 vial containing 6 mL of Stop Solution. (Caution! 1N H2SO4.
Handle with care.)
7. 1 vial containing 30 mL of Wash Solution concentrate 10X (PBST).
Needs to be diluted prior to use.
8. Instructions
