Cell Culture Medium
McCoy’s 5A medium Modified Basal Medium 5A medium with high
concentration of glucose
product description:
McCoy’s 5A medium is a medium designed by Thomas McCoy et al. in
1959, and it was originally specially used for the cultivation of
Novikoff Hepatoma cells. McCoy’s 5A medium is a modified Basal
Medium 5A. Unlike other media, McCoy’s 5A contains reduced
glutathione, back to-peptone, and high concentrations of glucose.
Product Usage:
McCoy’s 5A is widely used in the culture of various types of
primary cells, such as bone marrow, skin, gingiva, kidney, spleen,
lung, rat embryo, omentum, etc. In addition, McCoy’s 5A medium is
also used for tissue biopsy culture, cell line establishment, and
the culture of some lymphocytes and difficult-to-culture cells...
For a wide range of cell culture applications, we offer McCoy’s 5A
Modified Medium in various components.
| form | liquid |
| product name | McCoy’s 5A Cell Culture Medium |
| Specification | 500mL |
| place of origin | China |
| brand | HUACHENYANG |
| color | light red |
L-glutamine (Glutamine) is an essential nutrient element in cell
culture, but it is unstable in solution and will spontaneously
degrade. The medium without L-glutamine can be adjusted arbitrarily
according to research needs. - The amount of glutamine, adding
fresh L-glutamine or its substitutes is more conducive to the
growth of cells when using.
Phenol red is used as an indicator of pH value in the culture medium to
continuously monitor the pH of the culture medium. At low pH
values, phenol red makes the culture medium yellow, while at higher
pH values, it makes the culture medium purple. , when the pH value
is 7.2 to 7.4, it is red, which is most suitable for cell culture.
However, phenol red also has some disadvantages. Therefore, it is
best to use a medium without phenol red when using it. Phenol red
interferes with detection during flow cytometry analysis. In
addition, the presence of phenol red in some formulations of
serum-free media interferes with sodium-potassium balance.
Penicillin-streptomycin mixed solution is usually also called
"double antibody", which is the most commonly used antibiotic to
prevent microbial contamination in vitro culture. Among them,
penicillin can interfere with the synthesis of bacterial cell walls
and is particularly effective against Gram-positive bacteria; while
streptomycin Mycin can bind to the 30S subunit of bacterial
ribosomes and inhibit the synthesis of bacterial proteins. It is
effective against both Gram-negative bacteria and Gram-positive
bacteria but is particularly effective against Gram-negative
bacteria. The combination of penicillin and streptomycin can
prevent most bacterial contamination.
This product contains amino acids, vitamins, inorganic salts, and
other ingredients required for various types of cell culture, but
does not contain proteins, lipids, or any growth factors, so this
product should be used with serum or serum-free supplements.
Precautions:
This product is sterilized by filtration, and aseptic operation
should be paid attention to when using it to avoid contamination;
In order to maintain the best use effect of this product, do not
freeze or thaw;
This product is only used for scientific research or further
research, not for diagnosis and treatment.
