Human Insulin-like growth factor 1;IGF-1;Mechano growth factor;MGF
ELISA Kit
Usage: For research use only.
Cat. NO.In-Hu3783
For the quantitative in vitro determination ofIGF-1concentrations inHumanserum, plasma, culture media or any biological fluid.
Expiration date:6 months .
Storage: 2-8℃.
Package Size :96 wells/Kit
Principle
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa
stripplate provided in this kit has been pre-coated with an
antibody specific to IGF-1. Standards or samples are added to the
appropriate Microelisa stripplate wells and combined to the
specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated
antibody specific for IGF-1 is added to each Microelisa stripplate
well and incubated. Free components are washed away. The TMB
substrate solution is added to each well. Only those wells that
contain IGF-1 and HRP conjugated IGF-1 antibody will appear blue in
color and then turn yellow after the addition of the stop solution.
The optical density (OD) is measured spectrophotometrically at a
wavelength of 450 nm. The OD value is proportional to the
concentration of IGF-1. You can calculate the concentration of
IGF-1 in the samples by comparing the OD of the samples to the
standard curve.
Materials provided with the kit
Materials provided with the kit
48 determinations
96 determinations
Storage
1
User manual
1
1
R.T.
2
Closure plate membrane
2
2
R.T.
3
Sealed bags
1
1
R.T.
4
Microelisa stripplate
1
1
2-8℃
5
Standard:90ng/ml
0.5ml×1 bottle
0.5ml×1 bottle
2-8℃
6
Standard diluent
1.5ml×1 bottle
1.5ml×1 bottle
2-8℃
7
HRP-Conjugate reagent
3ml×1 bottle
6ml×1 bottle
2-8℃
8
Sample diluent
3ml×1 bottle
6ml×1 bottle
2-8℃
9
Chromogen Solution A
3ml×1 bottle
6ml×1 bottle
2-8℃
10
Chromogen Solution B
3ml×1 bottle
6ml×1 bottle
2-8℃
11
Stop Solution
3ml×1 bottle
6ml×1 bottle
2-8℃
12
Wash solution
20ml (20X)×1bottle
20ml (30X)×1bottle
2-8℃
Calculation of Results
Known concentrations of Human IGF-1 Standard and its corresponding
reading OD is plotted on the log scale (x-axis) and the log scale
(y-axis) respectively. The concentration of Human IGF-1 in sample
is determined by plotting the sample’s O.D. on the Y-axis. The
original concentration is calculated by multiplying the dilution
factor.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with
low, middle and high level Human IGF-1 were tested 20 times on one
plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with
low, middle and high level Human IGF-1were tested on 3 different
plates, 8 replicates in each plate.
Biovantion Inc is independent from international department of
Bioneovan Co., Ltd., which established in 2 0 0 5, is an in vitro
diagnostic reagents manufacturer engaged in the research,
development, production, and focus on clinical testing for
infectious diseases, such as Hepatitis virus, Noro virus, Parvo
virus test kits, our products include rapid test cassette and Elisa
kits.
All our products have been registered in National Medical Products
Administration of China, in year 2020 we get Certificate of ISO
13485:2016 – QUALITY MANAGEMENT SYSTEMS FOR MEDICAL DEVICES from
SGS. More importantly, we have awarded the registration certificate
from National Medical Products Administration for Diagnostic Kit
for IgM Antibody to COVID-19 and IgG antibody to COVID-19 during
the outbreak in China, and also we developed COVID-19 Antigen test
; Neutralizing Antibody Test, these products selling well all
overthe world
