Actinobacillus pleuropneumoniae(APP) Nucleic Acid Detection Kits
(PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the detection of DNA of Actinobacillus
pleuropneumoniae (APP) in tracheal secretions swabs, lung tissue
samples, etc., for the auxiliary diagnosis of Actinobacillus
pleuropneumoniae.
Principle:
This kit choose swine Actinobacillus pleuropneumoniae conservative
design specific gene probe, the probe can with primer amplification
area in the middle of a specific binding DNA template, in the
process of PCR extension, the circumscribed Taq polymerase enzymes
from the 5 'end of fluorescent groups on the probe will be cut
down, make it free in the reaction system, thereby quenching groups
out of the 3' end, Fluorescence was produced and detected by
fluorescence PCR instrument to achieve the detection of
actinobacillus pleuropneumoniae nucleic acid in the completely
closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 24 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Swab of tracheal secretion, lung tissue, etc
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
