Classical Swine Fever virus (CSFV) Nucleic Acid Detection Kits
(PCR-Fluorescent Probe Method)
Intended use:
This kit is used for diagnosis, monitoring and epidemiological
investigation of Classical swine fever virus (CSFV), and is
suitable for specific detection of CSFV in pig blood, viscera and
other tissue samples.
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Selects the swine
fever virus gene conservative design specific probe, the probe with
primer amplification area in the middle of a specific binding DNA
template, in the process of PCR extension, the circumscribed Taq
polymerase enzymes from the 5 'end of fluorescent groups on the
probe will be cut down, make it free in the reaction system,
thereby quenching groups out of the 3' end, glow, The nucleic acid
of CLASSICAL Swine fever virus (CSFV) was detected by fluorescence
PCR in a completely closed reaction system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Pig blood, viscera and other tissue samples
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
