Porcine Epidemic Diarrhea virus (PEDV) Nucleic Acid Detection Kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is used for diagnosis, monitoring and epidemiological
investigation of porcine epidemic diarrhea, and is suitable for
specific detection of porcine epidemic diarrhea virus (PEDV) in
small intestine and feces samples of pigs.
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Choose the pig
epidemic diarrhea virus gene conservative design specific probe,
the probe with primer amplification area in the middle of a
specific binding DNA template, in the process of
PCR extension, the circumscribed Taq polymerase enzymes from the 5
'end of fluorescent groups on the probe will be cut down, make it
free in the reaction system, thereby quenching groups out of the 3'
end, glow, The nucleic acid of porcine epidemic diarrhea virus was
detected by fluorescence PCR in a completely closed reaction
system.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Small intestine, feces and other samples of pigs
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -70℃.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value=0 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
