Rotavirus A (RVA)Nucleic Acid Detection Kit
(PCR-Fluorescent Probe Method)
Intended use:
This kit is suitable for the specific detection of pathogenic
rotavirus TYPE A (RVA) in porcine small intestine and fresh feces.
Principle:
This kit uses RNA as a template and primers as a starting point to
synthesize cDNA strands complementary to RNA template under the
action of high-efficiency reverse transcriptase. Choose the pig
epidemic diarrhea virus gene conservative design specific probe,
the probe with primer amplification area in the middle of a
specific binding DNA template, in the process of PCR extension, the
circumscribed Taq polymerase enzymes from the 5 'end of fluorescent
groups on the probe will be cut down, make it free in the reaction
system, thereby quenching groups out of the 3' end, glow, The
detection of rotavirus TYPE A (RVA) nucleic acid in A completely
closed reaction system was realized by fluorescence PCR instrument.
Main components:
| Component | Specification |
| PCR reaction liquid | 48 tubes |
| Positive control | 1 tube |
| Negative control | 1 tube |
Note: components in different batches of kits should not be
interchangeable.
Sample requirements:
1. Sample type:
Pig small intestine, fresh feces, etc
2. Sample collection and transportation:
Sample collection and transportation shall be carried out in
accordance with NY/T 541. Samples shall be transported to the
laboratory as soon as possible under refrigerated conditions to
avoid repeated freeze-thaw.
3. Sample preservation:
It can be stored for 24 hours at 2℃~8℃, and can be stored for a
long time at -20℃.
The results of interpretation:
| Results | Ct Value |
| Positive | Ct≤40 |
| Negative | No Ct value or Ct value≥45 |
| Gray area | 40＜Ct≤45 |
Note: The result is judged to be grey area, which needs to be
rechecked. If the recheck result is Ct value < 45 is considered
as positive, and no Ct value or Ct value ≥45 is considered as
negative.
